Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A nonuniform electrical field electroporation chamber design.

T Hollon1, F K Yoshimura

  • 1Department of Microbiology, University of Washington, Seattle 98195.

Analytical Biochemistry
|November 1, 1989
PubMed
Summary

A new, inexpensive electroporation cylinder design significantly improves gene transfection efficiency sixfold compared to standard electroporation cuvettes. This novel chamber design offers a powerful alternative for cell electroporation, especially when optimal electrical field strengths are unknown.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical trial design changing as molecular targets gain popularity.

Journal of the National Cancer Institute·2001
Same author

Comparing microarray data: What technology is needed?

Journal of the National Cancer Institute·2001
Same author

Mink cell focus-forming murine leukemia virus killing of mink cells involves apoptosis and superinfection.

Journal of virology·2001
Same author

Role of the LTR region between the enhancer and promoter in mink cell focus-forming murine leukemia virus pathogenesis.

Virology·2001
Same author

FDA uneasy about placebo revision.

Nature medicine·2001
Same author

HGS targets patent-expiring drugs.

Nature biotechnology·2000

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Background:

  • Electroporation is a common method for introducing molecules into cells.
  • Standard electroporation often uses uniform electrical fields, which may not be optimal for all cell types.
  • Improving transfection efficiency is crucial for various molecular and cell biology applications.

Purpose of the Study:

  • To introduce and evaluate an inexpensive electroporation cylinder design.
  • To compare the transfection efficiency of the electroporation cylinder with standard electroporation cuvettes.
  • To assess the impact of the new design on cell viability and transfection variability.

Main Methods:

  • Development of a novel electroporation cylinder creating a non-uniform electrical field.

Related Experiment Videos

  • Testing the electroporation cylinder and standard electroporation cuvettes in five mouse cell lines.
  • Utilizing a transient gene expression assay to measure transfection efficiency.
  • Assessing cell viability by comparing cell death percentages between the two designs.

    • Main Results:

    • The electroporation cylinder improved transfection efficiency approximately sixfold compared to electroporation cuvettes.
    • Both designs exhibited similar effects on the variability of transfection efficiency.
    • Electroporation cylinders delivered electrical field strengths comparable to cuvettes, as indicated by similar cell death rates.

    Conclusions:

    • The electroporation cylinder is an effective and inexpensive alternative to standard electroporation cuvettes.
    • This design enhances transfection efficiency, making it valuable for research.
    • The electroporation cylinder is particularly advantageous when optimal electrical field strengths are unknown or difficult to achieve.