Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lipid Catabolism01:25

Lipid Catabolism

1.4K
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reevaluation of mycobacterial Phosphatidyl-myo-Inositol Mannosides (PIMs) as Toll-Like Receptor 2 (TLR2) Agonists.

Journal of lipid research·2026
Same author

Conserved T cell receptor usage underpins recognition of CD1c presenting a mycobacterial lipid.

bioRxiv : the preprint server for biology·2026
Same author

Adjunctive ibuprofen in pre-extensively drug-resistant and extensively drug-resistant tuberculosis: a phase IIA open-label pilot clinical trial.

Nature communications·2026
Same author

CD1a-Mediated Presentation of Canonical Microbial Peptides to T Cells.

bioRxiv : the preprint server for biology·2026
Same author

Proteome of Bronchial Alveolar Lavage fluid and Serum from Tuberculosis patients during drug treatment.

Scientific data·2026
Same author

Optimized Mass Spectrometry to Uncover M. tuberculosis Biomarkers in Extracellular Vesicles from Asymptomatic Tuberculosis Patients.

The Journal of infectious diseases·2026

Related Experiment Video

Updated: Apr 8, 2026

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
07:42

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Published on: April 16, 2021

9.7K

Mycobacterial Lipidomics.

Emilie Layre1, Reem Al-Mubarak2, John T Belisle2

  • 1Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.

Microbiology Spectrum
|June 25, 2015
PubMed
Summary
This summary is machine-generated.

Lipidomics, the study of lipids, is crucial for understanding Mycobacterium tuberculosis virulence. This research explores mass spectrometry techniques to analyze the unique and diverse lipids found in this bacterium.

More Related Videos

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.9K
Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
07:56

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Published on: September 3, 2012

16.0K

Related Experiment Videos

Last Updated: Apr 8, 2026

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
07:42

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Published on: April 16, 2021

9.7K
Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.9K
Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
07:56

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Published on: September 3, 2012

16.0K

Area of Science:

  • Biochemistry
  • Microbiology
  • Analytical Chemistry

Background:

  • Lipidomics is a specialized field within metabolomics focusing on hydrophobic molecules like lipids.
  • Mycobacterium tuberculosis possesses unique and structurally diverse lipid classes, primarily found in Actinobacteria.
  • Understanding these lipids is key to unraveling the virulence mechanisms of this lipid-rich bacterium.

Purpose of the Study:

  • To outline the fundamental principles of lipidomics.
  • To discuss the practical challenges in applying mass spectrometry-based lipidomic studies to Mycobacterium tuberculosis.
  • To address key questions regarding the role of lipids in M. tuberculosis virulence.

Main Methods:

  • Mass spectrometry-based lipidomic analysis.
  • Characterization of lipid classes specific to Actinobacteria.
  • Investigating lipid structures and their implications for bacterial physiology.

Main Results:

  • Identification of unique lipid profiles in M. tuberculosis.
  • Highlighting the structural diversity of lipids within this pathogen.
  • Establishing a framework for future lipidomic investigations.

Conclusions:

  • Mass spectrometry is a powerful tool for M. tuberculosis lipidomics.
  • Further research into M. tuberculosis lipids can elucidate virulence factors.
  • Lipidomic studies are essential for understanding this important human pathogen.