Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

18.6K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
18.6K
Transposons01:24

Transposons

3.1K
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
3.1K
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

21.2K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
21.2K
LTR Retrotransposons03:08

LTR Retrotransposons

20.5K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
20.5K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

14.1K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
14.1K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

11.9K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
11.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Educational, judicial, and social outcomes of children exposed to intimate partner violence.

Child abuse & neglect·2026
Same author

A Small RNA Derived From the 5' End of the IS200 tnpA Transcript Regulates Multiple Virulence Regulons in Salmonella typhimurium.

Molecular microbiology·2025
Same author

Placental small extracellular vesicles from normal pregnancy and gestational diabetes increase insulin gene transcription and content in β cells.

Clinical science (London, England : 1979)·2024
Same author

Identification of high-performing antibodies for the reliable detection of Tau proteoforms by Western blotting and immunohistochemistry.

Acta neuropathologica·2024
Same author

A platform for predicting mechanism of action based on bacterial transcriptional responses identifies an unusual DNA gyrase inhibitor.

Cell reports·2024
Same author

Incidence of Subsequent Mental Health Disorders and Social Adversity Following Pediatric Concussion: A Longitudinal, Population-Based Study.

The Journal of pediatrics·2023

Related Experiment Video

Updated: Apr 8, 2026

Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity
04:04

Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

Published on: January 20, 2023

2.9K

Transposons Tn10 and Tn5.

David B Haniford1, Michael J Ellis1

  • 1Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada.

Microbiology Spectrum
|June 25, 2015
PubMed
Summary
This summary is machine-generated.

Bacterial transposons Tn10 and Tn5 regulation by H-NS and Hfq proteins is explored. H-NS upregulates transposition, while Hfq downregulates it, linking gene transfer to environmental cues.

More Related Videos

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

11.6K
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
13:31

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

14.6K

Related Experiment Videos

Last Updated: Apr 8, 2026

Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity
04:04

Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

Published on: January 20, 2023

2.9K
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

11.6K
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
13:31

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

14.6K

Area of Science:

  • Molecular Biology
  • Microbiology
  • Genetics

Background:

  • Bacterial transposons Tn10 and Tn5 are key models for DNA transposition.
  • Research focuses on host regulation and synthetic biology applications.
  • H-NS and Hfq proteins are identified as novel regulators of Tn10 and Tn5.

Purpose of the Study:

  • To investigate the roles of H-NS and Hfq proteins in regulating Tn10 and Tn5 transposition.
  • To explore the connection between these transposon systems, antibiotic resistance gene transfer, and environmental cues.
  • To summarize recent advancements in using Tn10 antisense RNA for synthetic biology.

Main Methods:

  • Analysis of nonreplicative DNA transposition mechanisms.
  • Study of transpososome dynamics and structure.
  • Investigation of host regulatory proteins (H-NS and Hfq) and their impact on transposition.

Main Results:

  • H-NS protein upregulates both Tn10 and Tn5 transposition by acting on transposition complexes.
  • Hfq protein downregulates both systems by affecting transposase expression.
  • Tn10 antisense RNA is being incorporated into riboswitches for synthetic biology.

Conclusions:

  • H-NS and Hfq proteins provide a link between bacterial transposons, gene transfer, and environmental sensing.
  • Understanding these regulatory mechanisms is crucial for controlling antibiotic resistance.
  • Tn10-based riboregulators show promise for synthetic biology applications.