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Shikonin inhibits invasiveness of osteosarcoma through MMP13 suppression

Biyong Deng1, Bing Qiu2

  • 1Department of Orthopedics, Guizhou Province Osteological Hospital, 25 Shachong S Rd, Guiyang, 550007, China.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
|June 25, 2015
PubMed

Insights

Shikonin, a compound from Chinese herbs, reduces osteosarcoma (OS) cell invasiveness by targeting matrix metalloproteinase-13 (MMP-13). This suggests shikonin could be a potential therapeutic agent against OS metastasis.

Area of Science:

  • Oncology
  • Pharmacology
  • Biochemistry

Background:

  • Osteosarcoma (OS) is a primary bone cancer known for metastasis.
  • Shikonin, a herbal constituent, induces necroptosis in OS cells.
  • The effect of low-dose shikonin on OS cell invasiveness remains uninvestigated.

Purpose of the Study:

  • To investigate the effects of shikonin on osteosarcoma cell invasiveness.
  • To identify the molecular target of shikonin's anti-invasive action in OS.

Main Methods:

  • Scratch wound healing assay and transwell cell migration assay were used to assess cell invasiveness.
  • The role of matrix metalloproteinase (MMP)-13 was evaluated.
  • Experiments were conducted with MMP13-overexpressing OS cells.

Main Results:

  • Shikonin demonstrated a dose-dependent decrease in OS cell invasiveness.
  • Matrix metalloproteinase (MMP)-13 was identified as a direct target of shikonin's inhibitory effect on invasiveness.
  • The suppressive effect of shikonin on OS cell invasiveness was abrogated in cells overexpressing MMP13.

Conclusions:

  • Shikonin suppresses osteosarcoma cell invasiveness, potentially through the inhibition of MMP-13.
  • These findings highlight a novel role for shikonin in mitigating OS cell metastasis.
Keywords:
Matrix metalloproteinase (MMP)-13Osteosarcoma (OS)Shikonin

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