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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis
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Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis

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Mycobacterium tuberculosis in the Proteomics Era.

Martin Gengenbacher1, Jeppe Mouritsen2, Olga T Schubert2

  • 1Max Planck Institute for Infection Biology, Department of Immunology, Charitéplatz 1, 10117 Berlin, Germany.

Microbiology Spectrum
|June 25, 2015
PubMed
Summary

Proteomics has advanced understanding of Mycobacterium tuberculosis. New mass spectrometry techniques enable precise protein detection and quantification, aiding tuberculosis research and interventions.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Immunology

Background:

  • Proteomics has significantly enhanced the understanding of Mycobacterium tuberculosis (M. tuberculosis) over the past 20 years.
  • Early research utilized 2D gel electrophoresis and mass spectrometry (MS) to analyze M. tuberculosis proteomes, including subfractions like cell walls and secreted proteins.

Purpose of the Study:

  • To provide a comprehensive overview of mycobacterial proteome research and its key findings.
  • To explore proteins involved in M. tuberculosis intracellular survival and host cell manipulation under various conditions.
  • To characterize hypothetical proteins and posttranslational modifications in M. tuberculosis.

Main Methods:

  • Two-dimensional gel electrophoresis coupled with mass spectrometry (MS).
  • Analysis of proteomic composition under different culture conditions (acidic pH, nutrient starvation, low oxygen).
  • Application of advanced MS techniques like selected reaction monitoring (SRM) and data-independent acquisition (DIA).

Main Results:

  • Identification of proteins relevant to M. tuberculosis intracellular survival and host interaction.
  • Characterization of hypothetical proteins and posttranslational modifications.
  • Development of reliable methods for detecting and quantifying M. tuberculosis proteins using advanced MS and public databases.

Conclusions:

  • Proteomics offers powerful tools for understanding M. tuberculosis.
  • Advanced MS techniques enable precise protein analysis within host cells.
  • Proteomics holds significant potential for tuberculosis vaccinology, drug discovery, and biomarker identification.