Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling.

Drug discovery today·2016
Same author

A tunable hydrogel system for long-term release of cell-secreted cytokines and bioprinted in situ wound cell delivery.

Journal of biomedical materials research. Part B, Applied biomaterials·2016
Same author

Re: A Tumor Mitochondria Vaccine Protects against Experimental Renal Cell Carcinoma.

The Journal of urology·2016
Same author

Re: Endogenous Formaldehyde is a Hematopoietic Stem Cell Genotoxin and Metabolic Carcinogen.

The Journal of urology·2016
Same author

Re: Sphaeropsidin A Shows Promising Activity against Drug-Resistant Cancer Cells by Targeting Regulatory Volume Increase.

The Journal of urology·2016
Same author

Re: Sex Hormone-Dependent tRNA Halves Enhance Cell Proliferation in Breast and Prostate Cancers.

The Journal of urology·2016

Related Experiment Video

Updated: Apr 8, 2026

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
05:20

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Published on: February 6, 2020

8.5K

Bioengineered multilayered human corneas from discarded human corneal tissue.

Zhihua Zhang1, Guoguang Niu, Jin San Choi

  • 1Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC 27157, USA. Department of Ophthalmology, Shanghai First People's Hospital Affiliated to Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, People's Republic of China.

Biomedical Materials (Bristol, England)
|June 25, 2015
PubMed
Summary
This summary is machine-generated.

Bioengineered corneas created from discarded human eye tissue offer a promising solution to the global shortage of donor corneas for transplantation. These novel constructs show potential for improving vision and aiding ophthalmological research.

More Related Videos

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
07:35

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Published on: January 24, 2018

9.4K
A Human Corneal Organ Culture Model of Descemet's Stripping Only with Accelerated Healing Stimulated by Engineered Fibroblast Growth Factor 1
12:36

A Human Corneal Organ Culture Model of Descemet's Stripping Only with Accelerated Healing Stimulated by Engineered Fibroblast Growth Factor 1

Published on: July 22, 2022

5.2K

Related Experiment Videos

Last Updated: Apr 8, 2026

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
05:20

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Published on: February 6, 2020

8.5K
Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
07:35

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Published on: January 24, 2018

9.4K
A Human Corneal Organ Culture Model of Descemet's Stripping Only with Accelerated Healing Stimulated by Engineered Fibroblast Growth Factor 1
12:36

A Human Corneal Organ Culture Model of Descemet's Stripping Only with Accelerated Healing Stimulated by Engineered Fibroblast Growth Factor 1

Published on: July 22, 2022

5.2K

Area of Science:

  • Ophthalmology
  • Biomaterials Science
  • Tissue Engineering

Background:

  • Corneal transplantation restores vision but faces a global shortage of donor tissue.
  • Bioengineering offers a solution by utilizing discarded human corneas for cell and scaffold sourcing.

Purpose of the Study:

  • To fabricate multilayered corneal equivalents for full-thickness transplantation.
  • To address the limited supply of high-quality donor corneas.

Main Methods:

  • Human corneal endothelial cells (hCEC) and limbal epithelial cells (hLEC) were isolated from discarded corneas and expanded in vitro.
  • Corneal scaffolds were created via decellularization of human corneal stroma.
  • Scaffolds were seeded with hCEC and hLEC on both sides using specialized equipment.

Main Results:

  • Cell-seeded constructs supported hCEC and hLEC growth and organization for 2 weeks in vitro.
  • Immunohistochemistry confirmed the expression of specific cell markers.
  • The engineered corneas demonstrated greater transparency compared to acellular scaffolds.

Conclusions:

  • Multilayered corneal equivalents can be successfully fabricated from human donor-derived materials.
  • These bioengineered corneas show potential for transplantation and ophthalmological drug testing.