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B7-H3 expression in children with asthma exacerbation
Zhengrong Chen1, Guangbo Zhang, Yuqing Wang
1Department of Respiratory Disease, Soochow University Affiliated Children's Hospital, Soochow University, Suzhou, China.
Insights
Elevated B7-H3 levels are found in children with asthma exacerbation, suggesting its role in the condition. B7-H3 may serve as a valuable biomarker for assessing asthma exacerbations in pediatric patients.
Area of Science:
- Immunology
- Pediatric Pulmonology
- Biomarker Discovery
Background:
- B7-H3, a member of the B7 family, is implicated in asthma development.
- Understanding B7-H3's role in pediatric asthma exacerbations is crucial.
Purpose of the Study:
- To investigate the expression levels of B7-H3 in children experiencing asthma exacerbation.
- To correlate B7-H3 levels with clinical parameters and cytokine profiles in pediatric asthma.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was used to measure B7-H3 and cytokine levels (IFN-γ, IL-4, IL-10).
- Clinical data, including FEV(1) and fractional exhaled nitric oxide, were collected from 21 children with asthma exacerbation and 18 controls.
- B7-H3 levels were compared between asthmatic children and controls, and in relation to treatments like inhaled corticosteroids and prednisone.
Main Results:
- Children with asthma exacerbation exhibited significantly higher B7-H3 levels compared to controls.
- Asthma exacerbation was associated with elevated IL-4 and reduced IFN-γ levels.
- B7-H3 levels were lower in patients receiving inhaled corticosteroids and decreased after prednisone treatment, showing an inverse correlation with IFN-γ.
Conclusions:
- B7-H3 plays a significant role in pediatric asthma exacerbation.
- B7-H3 demonstrates potential as a clinical biomarker for evaluating the severity of asthma exacerbations in children.
Background:
Recent studies have shown that B7-H3, a recently identified B7 family member, plays a critical role in the development of asthma.
Objective:
This study is to explore the expression of B7-H3 in children with asthma exacerbation.
Methods:
Twenty-one Chinese children with asthma exacerbation as well as 18 nonasthmatic control Chinese children were enrolled. B7-H3 level and cytokines (interferon [IFN]-γ, interleukin [IL]-4, and IL-10) determination were performed by enzyme-linked immunosorbent assay (ELISA) technique. Meanwhile, clinical parameters including laboratory findings, forced expiratory volume in one second (FEV(1)) and fractional exhaled nitric oxide were obtained.
Results:
Children with asthma exacerbation had significantly higher levels of B7-H3 than controls (4.46 ± 1.33 versus 3.42 ± 1.48 ng/mL; p = 0.027). Plasma IL-4 level was significantly higher in asthma exacerbation subjects than controls (157.98 ± 21.57 versus 121.92 ± 24.37 pg/mL; p < 0.0001), and IFN-γ level was significantly lower in asthma exacerbation subjects (292.73 ± 152.47 versus 421.78 ± 145.84 pg/mL; p = 0.0107). Level of B7-H3 in asthma exacerbation subjects with inhaled corticosteroid (ICS) treatment recently was significantly lower than subjects without ICS treatment (t = 2.706; p = 0.0136). Additionally, levels of B7-H3 decreased remarkably after prednisone treatment. Level of sB7-H3 in asthma exacerbation subjects was inversely correlated with level of IFN-γ (r(p) = -0.605; p = 0.005) after adjustment.
Conclusion:
B7-H3 may play an important role in asthma exacerbation and was a useful clinical biomarker to evaluate asthma exacerbation.
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