Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Application of voxel-wise SAR efficiency to evaluate suitability of novel materials in MRI with parallel transmit.

Physics in medicine and biology·2026

Same author

Ultra-Wideline 2D Correlations Among Low-γ Species in Solid-State NMR via the Progressive Saturation of a Common Proton Reservoir.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same author

On the effects of hyperpolarized water-based dissolution on the solute and solvent <sup>1</sup>H NMR spectra of small molecules.

Physical chemistry chemical physics : PCCP·2026

Same author

14.1 T Liquid-State <sup>19</sup>F Overhauser Dynamic Nuclear Polarization in an Analytical Organic Setting.

Journal of the American Chemical Society·2026

Same author

Assessing the treatment of pancreatic ductal adenocarcinoma by deuterium metabolic imaging: a preclinical study.

Magma (New York, N.Y.)·2026

Same author

Timing Is Everything: How Subtle Timing Changes in MRI Echo Planar Imaging Can Significantly Alter Mechanical Vibrations and Sound Level.

Magnetic resonance in medicine·2026

Search research articles

Related Experiment Video

Updated: Apr 8, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Interleaved multishot imaging by spatiotemporal encoding: A fast, self-referenced method for high-definition

Rita Schmidt¹, Amir Seginer¹, Lucio Frydman¹

¹Chemical Physics Department, Weizmann Institute of Science, Rehovot, Israel.

Magnetic Resonance in Medicine

|June 26, 2015

Summary

An interleaved spatiotemporal encoding (SPEN) method offers improved imaging by correcting motion and artifacts. This advanced technique enables high-definition functional and diffusion MRI in challenging environments.

Keywords:

full refocusing high-definition fMRI high-resolution ADCs interleaved MRI referenceless reconstruction spatiotemporal encoding

More Related Videos

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Published on: September 30, 2021

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Related Experiment Videos

Last Updated: Apr 8, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Published on: September 30, 2021

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Area of Science:

Magnetic Resonance Imaging (MRI)
Image Reconstruction
Biomedical Engineering

Background:

Single-shot imaging by spatiotemporal encoding (SPEN) offers artifact immunity compared to echo planar imaging.
Further enhancements in resolution and signal-to-noise ratio are possible by moving beyond single-scan limitations.

Purpose of the Study:

To develop and evaluate an interleaved SPEN sequence for improved image quality.
To explore the potential of a referenceless correction algorithm for optimized image acquisition.

Main Methods:

Exploited SPEN's lack of aliasing during undersampling for interleaved shot segmentation.
Implemented a navigator-free image reconstruction analysis for automatic correction of inaccuracies.
Accounted for phase noises and object motion during signal acquisition.

Main Results:

The interleaved SPEN method produced high-quality images in phantoms and human volunteers, even in challenging environments.
Achieved submillimeter functional MRI activation maps localized to gray matter.
Obtained submillimeter diffusion coefficient maps of human brains.

Conclusions:

Developed an interleaved SPEN approach for high-definition image acquisition.
This technique shows promise for expanded functional and diffusion MRI applications.
Enables advanced imaging in inhomogeneous or mobile environments.