Human chromosome banding with restriction endonucleases HaeIII, HinfI and PstI

Human chromosome banding was carried out with restriction endonucleases (REs) HaeIII, HinfI, PstI and the effects of some factors on it were examined. HaeIII induced C and G banding patterns, HinfI induced negative C bands but the centromeric heterochromatin of chromosomes 3 and 4 remained selectively dark-stained. A new heteromorphism of C banded region was discovered in chromosome 4. PstI induced G-like banding pattern. The duration of enzymatic digestion, the concentration of glycerol in the reaction buffer, as well as the ageing and heating of chromosome preparations all had a significant influence on the banding effects of the REs.