Related Experiment Video
Updated: Apr 8, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
Published on: February 27, 2016
Nuclear respiratory factor 2 induces SIRT3 expression
F Kyle Satterstrom1,2, William R Swindell3, Gaëlle Laurent2
1Harvard School of Engineering and Applied Sciences, Cambridge, MA, 02138, USA.
Abstract:
The mitochondrial deacetylase SIRT3 regulates several important metabolic processes. SIRT3 is transcriptionally upregulated in multiple tissues during nutrient stresses such as dietary restriction and fasting, but the molecular mechanism of this induction is unclear. We conducted a bioinformatic study to identify transcription factor(s) involved in SIRT3 induction. Our analysis identified an enrichment of binding sites for nuclear respiratory factor 2 (NRF-2), a transcription factor known to play a role in the expression of mitochondrial genes, in the DNA sequences of SIRT3 and genes with closely correlated expression patterns. In vitro, knockdown or overexpression of NRF-2 modulated SIRT3 levels, and the NRF-2α subunit directly bound to the SIRT3 promoter. Our results suggest that NRF-2 is a regulator of SIRT3 expression and may shed light on how SIRT3 is upregulated during nutrient stress.
Related Concept Videos
Regulation of Nuclear Protein Sorting
Transducer Mechanism: Nuclear Receptors
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
Directionality of Nuclear Transport
Nuclear Export
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
The Nucleolus
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...