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Circulating resistin protein and mRNA concentrations and clinical severity of coronary artery disease
Jelena Joksic1, Miron Sopic1, Vesna Spasojevic-Kalimanovska1
1Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Insights
Plasma resistin is elevated in acute coronary syndrome (ACS) patients, correlating with disease severity. While resistin mRNA levels in peripheral blood mononuclear cells (PBMC) did not differ, both plasma resistin and PBMC resistin mRNA negatively correlated with HDL-C.
Area of Science:
- Biochemistry
- Cardiology
- Molecular Biology
Background:
- Previous research indicates a strong association between plasma resistin and coronary artery disease (CAD).
- Understanding resistin's role in different clinical severities of CAD is crucial for targeted therapeutic strategies.
Purpose of the Study:
- To investigate differences in plasma resistin protein and peripheral blood mononuclear cell (PBMC) resistin mRNA levels across varying clinical severities of CAD.
- To explore correlations between resistin levels, HDL-C, and serum creatinine in CAD patients.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) for plasma resistin.
- Real-time quantitative polymerase chain reaction (RT-qPCR) for PBMC resistin mRNA.
- Comparative analysis between healthy controls (CG), CAD-negative patients, and CAD-positive patients (stable angina pectoris [SAP] and acute coronary syndrome [ACS]).
Main Results:
- Plasma resistin was significantly higher in ACS patients compared to CG and CAD-negative groups.
- Resistin mRNA expression in PBMCs did not significantly differ across groups but correlated positively with plasma resistin.
- Both plasma resistin and PBMC resistin mRNA showed a negative correlation with HDL-C, and plasma resistin positively correlated with serum creatinine.
Conclusions:
- Elevated plasma resistin is a marker for acute coronary syndrome (ACS).
- A positive association exists between plasma resistin and resistin mRNA in PBMCs, despite unchanged mRNA levels across disease states.
- Resistin levels are inversely related to HDL-C and directly related to serum creatinine, suggesting potential roles in CAD pathogenesis and progression.
Introduction:
Previous studies have implicated a strong link between circulating plasma resistin and coronary artery disease (CAD). The aim of this study was to evaluate the differences in peripheral blood mononuclear cells (PBMC) resistin mRNA and its plasma protein concentrations between the patients with CAD of different clinical severity.
Material And Methods:
This study included 33 healthy subjects as the control group (CG) and 77 patients requiring coronary angiography. Of the latter 30 was CAD negative whereas 47 were CAD positive [18 with stable angina pectoris (SAP) and 29 with acute coronary syndrome (ACS)]. Circulating resistin was measured by ELISA; PBMC resistin mRNA was determined by real-time PCR.
Results:
Resistin protein was significantly higher in the ACS group compared to the CG (P=0.001) and the CAD negative group (P=0.018). Resistin mRNA expression did not vary across the study groups, despite the positive correlation seen with plasma resistin (ρ=0.305, P=0.008). In patients, plasma resistin and PBMC resistin mRNA negatively correlated with HDL-C (ρ=-0.404, P<0.001 and ρ=-0.257, P=0.032, respectively). Furthermore, the highest plasma resistin tertile showed the lowest HDL-C (P=0.006). Plasma resistin was positively associated with serum creatinine (ρ=0.353, P=0.002).
Conclusion:
Significant increase of plasma resistin in patients with ACS compared to CG and CAD negative patients was observed. Despite no change in PBMC resistin mRNA in different disease conditions a positive association between resistin mRNA and resistin plasma protein was evident. Both plasma resistin and PBMC resistin mRNA were negatively associated with plasma HDL-C, and plasma resistin positively with serum creatinine.
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