Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Complete sequencing of medaka genomes reveals the architecture of centromeric satellites, giant mobile elements, and sex chromosomes.

Genome research·2026
Same author

Tracing genome size dynamics in sharks and rays with inclusive sequence analysis by the Squalomix Consortium.

Genome research·2026
Same author

The Greenland shark genome: Insights into lifespan extremes and population dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

ATAC-seq of low-input and cryopreserved primordial germ cells reveals functional enhancers.

Development (Cambridge, England)·2026
Same author

MIFE and MIFD: Minimum information for fermentation experiments and devices.

GigaScience·2026
Same author

Design of Neuronal Supramolecular Scaffolds Integrating Cell Signaling and Electrical Conductivity.

ACS biomaterials science & engineering·2026
Same journal

ECHIDNA: Extreme Climate Historical and Future Indices Data under Numerous Approaches across Major Chinese River Basins Based on CMIP6 Multi-Model Ensemble.

Scientific data·2026
Same journal

An open fMRI dataset examining the effects of online social and non-social information distraction on attention.

Scientific data·2026
Same journal

A comprehensive dataset of 32 million pentapeptide structures for high-throughput virtual screening.

Scientific data·2026
Same journal

A Canadian Benchmark LiDAR Dataset for Urban Infrastructure and 3D Scene Understanding.

Scientific data·2026
Same journal

A Chromosome-Level Genome Assembly of Sitotroga cerealella (Olivier, 1789) (Lepidoptera: Gelechiidae), a Global Pest of Stored Grains.

Scientific data·2026
Same journal

The Dataset of Daily Air Quality for the Years 2013-2023 in Italy.

Scientific data·2026
See all related articles

Related Experiment Video

Updated: Apr 8, 2026

Whole-mount Retinal Organoid Visualization with Cellular Resolution
09:20

Whole-mount Retinal Organoid Visualization with Cellular Resolution

Published on: June 20, 2025

1.8K

Comparative, transcriptome analysis of self-organizing optic tissues.

Munazah Andrabi1, Shigehiro Kuraku1, Nozomu Takata2

  • 1Phyloinformatics Unit, RIKEN: Center for Life Science Technologies , Kobe 650-0047, Japan.

Scientific Data
|June 26, 2015
PubMed
Summary
This summary is machine-generated.

This study reveals how Fgf and Wnt/β-catenin signaling pathways influence gene expression during optic cup development in vitro. Understanding these molecular mechanisms aids in optimizing stem cell-based retinal tissue engineering.

More Related Videos

En face Cryosectioning of Mouse Retina for High-dimensional Spatial Molecular Analysis
08:57

En face Cryosectioning of Mouse Retina for High-dimensional Spatial Molecular Analysis

Published on: July 8, 2025

1.6K
Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
08:17

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

Published on: September 22, 2017

20.4K

Related Experiment Videos

Last Updated: Apr 8, 2026

Whole-mount Retinal Organoid Visualization with Cellular Resolution
09:20

Whole-mount Retinal Organoid Visualization with Cellular Resolution

Published on: June 20, 2025

1.8K
En face Cryosectioning of Mouse Retina for High-dimensional Spatial Molecular Analysis
08:57

En face Cryosectioning of Mouse Retina for High-dimensional Spatial Molecular Analysis

Published on: July 8, 2025

1.6K
Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
08:17

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

Published on: September 22, 2017

20.4K

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Genomics

Background:

  • Embryonic stem cells self-organize into optic cups in vitro using SFEBq culture.
  • Neural retina epithelial tissues use Fgf and Wnt/β-catenin signaling for differentiation into neural retina and retinal-pigmented epithelium.

Purpose of the Study:

  • To investigate the impact of Fgf and Wnt/β-catenin signaling on gene expression during optic tissue formation.
  • To provide a transcriptome-scale dataset for understanding optic tissue differentiation.

Main Methods:

  • Generated Rx+ optic tissue using the SFEBq method.
  • Applied Fgf or Wnt/β-catenin stimulation to the cultured optic tissues.
  • Analyzed gene expression changes over time using RNA-Sequencing (RNA-Seq).

Main Results:

  • Generated a comparative transcriptomic dataset detailing gene expression under different signaling pathway activations.
  • Identified key gene expression alterations induced by Fgf and Wnt/β-catenin signaling.

Conclusions:

  • The study elucidates signaling pathway-driven gene expression during in vitro optic tissue development.
  • Findings will inform optimization of stem cell culture techniques for retinal tissue engineering.