Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biomedical Concept Recognition with Error-aware Negative-enhanced Ranking Framework.

Bioinformatics (Oxford, England)·2026
Same author

Control efficacy of Bt-(Cry1Ab + Cry1F) maize against Spodoptera frugiperda (Lepidoptera: Noctuidae) immigrant populations in North China.

Pest management science·2026
Same author

Spatially explicit temperature optima improve climate impact assessment of global crop productivity.

Nature communications·2026
Same author

Protein Tyrosine Phosphatase 1B-Mediated Granulosa Cell Insulin Resistance Links Metabolic Stress to Aging-Relevant Ovarian Dysfunction and Is Reversed by Gengnianchun.

Aging cell·2026
Same author

Advancing the identification of toxic by-products in disinfected water by integrating effect-directed analysis and nontarget screening.

Journal of hazardous materials·2026
Same author

Single-Layer Narrow-Bandgap Co-Doped Ti<sub>2</sub>CO<sub>2</sub> MXene as a Multifunctional Cocatalyst for Scalable and Stable BiVO<sub>4</sub> Photoanodes.

ChemSusChem·2026

Related Experiment Video

Updated: Apr 8, 2026

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs
07:22

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs

Published on: November 20, 2018

8.1K

Evaluation of Appropriate Reference Genes for Gene Expression Normalization during Watermelon Fruit Development.

Qiusheng Kong1, Jingxian Yuan1, Lingyun Gao1

  • 1Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

Plos One
|June 26, 2015
PubMed
Summary

This study identifies reliable reference genes for accurate gene expression analysis in watermelon fruits. Validated genes like clathrin adaptor complex subunit (ClCAC) improve understanding of fruit development and quality traits.

More Related Videos

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
11:04

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Published on: November 30, 2015

14.1K
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
06:31

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

10.4K

Related Experiment Videos

Last Updated: Apr 8, 2026

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs
07:22

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs

Published on: November 20, 2018

8.1K
Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
11:04

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Published on: November 30, 2015

14.1K
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
06:31

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

10.4K

Area of Science:

  • Plant Molecular Biology
  • Fruit Development and Physiology
  • Biotechnology

Background:

  • Accurate gene expression analysis is crucial for understanding watermelon (Citrullus lanatus) fruit development and quality.
  • Real-time quantitative reverse-transcription PCR (qRT-PCR) is a standard method, but requires validated reference genes for normalization.
  • Reference gene stability in watermelon fruit under various conditions remains poorly characterized.

Purpose of the Study:

  • To evaluate the suitability of 12 candidate genes as reference genes for qRT-PCR analysis in watermelon fruits.
  • To identify the most stable reference genes across different genotypes, fruit setting methods, and developmental stages.
  • To establish reliable normalization controls for gene expression studies in watermelon fruit biology.

Main Methods:

  • Expression levels of 12 candidate reference genes were analyzed using qRT-PCR.
  • Samples included 48 watermelon fruits from 12 developmental stages, covering two genotypes and two fruit setting methods (parthenocarpic and fertilized).
  • Statistical algorithms geNorm and NormFinder were employed to assess gene expression stability.

Main Results:

  • geNorm identified clathrin adaptor complex subunit (ClCAC), β-actin (ClACT), and alpha tubulin 5 (ClTUA5) as the most stable multiple reference genes.
  • NormFinder ranked ClCAC as the best single reference gene, and ClCAC with SAND family protein (ClSAND) as the top two reference genes.
  • Normalization using validated reference genes revealed a strong correlation between phytoene synthase (ClPSY1) expression and lycopene accumulation during fruit ripening.

Conclusions:

  • ClCAC, ClACT, ClTUA5, and ClSAND are validated as reliable reference genes for gene expression studies in watermelon fruits.
  • These findings provide essential tools for accurate transcriptomic analysis in watermelon.
  • The validated reference genes will advance research into watermelon fruit biology, development, and quality improvement.