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Effects of thiopentone on immunoglobulin production in vitro
1Department of Anaesthesiology, University Central Hospital, Turku, Finland.
Thiopentone is known to depress several granulocyte and lymphocyte functions. This study assessed the effects of thiopentone on IgG, IgM and IgA production by pokeweed mitogen-activated lymphocytes as a measure of B-lymphocyte function. Thiopentone decreased IgG production at greater than or equal to 25 micrograms ml-1 (91 mumol litre-1) concentrations and that of IgM and IgA at greater than or equal to 50 micrograms ml-1 (182 mumol litre-1) concentrations over 7 days of culture. Preincubation of lymphocytes for 1 h with thiopentone 0-500 micrograms ml-1 (0-1820 mumol litre-1) had no effect on immunoglobulin production, whereas incubation for 2 and 3 h decreased IgG and IgM production with toxic thiopentone concentrations of 500 micrograms ml-1. Hydrocortisone-sensitive suppressor cells and concanavalin A-inducible suppressor cells were more sensitive to high concentrations of thiopentone than the other cell populations. These findings may be important in patients receiving barbiturate treatment for cerebral protection.
Thiopentone is known to depress several granulocyte and lymphocyte functions. This study assessed the effects of thiopentone on IgG, IgM and IgA production by pokeweed mitogen-activated lymphocytes as a measure of B-lymphocyte function. Thiopentone decreased IgG production at greater than or equal to 25 micrograms ml-1 (91 mumol litre-1) concentrations and that of IgM and IgA at greater than or equal to 50 micrograms ml-1 (182 mumol litre-1) concentrations over 7 days of culture. Preincubation of lymphocytes for 1 h with thiopentone 0-500 micrograms ml-1 (0-1820 mumol litre-1) had no effect on immunoglobulin production, whereas incubation for 2 and 3 h decreased IgG and IgM production with toxic thiopentone concentrations of 500 micrograms ml-1. Hydrocortisone-sensitive suppressor cells and concanavalin A-inducible suppressor cells were more sensitive to high concentrations of thiopentone than the other cell populations. These findings may be important in patients receiving barbiturate treatment for cerebral protection.