Registered report: BET bromodomain inhibition as a therapeutic strategy to target c-Myc

Area of Science:

• Oncology
• Molecular Biology
• Cancer Therapeutics

Background:

• Reproducibility in scientific research is a growing concern.
• The Reproducibility Project: Cancer Biology aims to replicate key findings from high-impact cancer studies.
• This report details the replication plan for experiments from Delmore et al., 2011, published in Cell.

Purpose of the Study:

• To replicate in vitro and in vivo experiments on BET bromodomain inhibition targeting the c-Myc oncogene.
• To validate the therapeutic potential of JQ1 in cancer models.
• To contribute to the growing body of evidence on the reproducibility of cancer biology research.

Main Methods:

• Replication of experiments reported in Figure 3B (in vitro) and Figures 7C-E (in vivo) from Delmore et al., 2011.
• Utilizing JQ1, a small molecule inhibitor of BET bromodomains.
• Assessing transcriptional down-regulation of c-Myc and evaluating tumor burden and survival in a multiple myeloma mouse model.

Main Results:

• Delmore et al. (2011) demonstrated that JQ1 down-regulates c-Myc expression in vitro.
• Delmore et al. (2011) showed that JQ1 treatment reduced tumor burden and increased survival in mice with multiple myeloma.
• This registered report outlines the planned replication of these specific findings.

Conclusions:

• The replication of these key experiments will provide valuable data on the robustness of findings regarding BET bromodomain inhibitors in cancer biology.
• Successful replication would support JQ1 as a potential therapeutic strategy for cancers involving c-Myc.
• The results will be published in eLife, contributing to open science and research reproducibility.