Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Architecture and function of holocentric CENP-A-independent inner kinetochores.

Science advances·2026
Same author

Direct RNA Sequencing reveals epitranscriptomic regulation of brain cells and Alzheimer's Disease pathology.

bioRxiv : the preprint server for biology·2026
Same author

Antibody-Mediated Delivery of BRM/BRG1 Protein Degraders Affords Strong Antitumor Efficacy in Multiple BRM-Dependent Non-Small Cell Lung Cancer Xenograft Models.

Journal of medicinal chemistry·2026
Same author

Phosphorylated ubiquitin is a secondary messenger and an epigenetic mark mediating mitochondria to nucleus signaling.

bioRxiv : the preprint server for biology·2026
Same author

From Congestion to Clarity: On the Complementarity of Resolving Power and Spectral Simplification for Intact Protein Characterization.

Journal of the American Society for Mass Spectrometry·2026
Same author

Template-driven scaffolding of SCF<sup>FBXO42</sup> regulates PP2A degradation.

Nature·2026

Related Experiment Video

Updated: Apr 8, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

10.2K

A calibration routine for efficient ETD in large-scale proteomics.

Christopher M Rose1,2, Matthew J P Rush1,2, Nicholas M Riley1,2

  • 1Department of Chemistry, University of Wisconsin, Madison, WI, 53706, USA.

Journal of the American Society for Mass Spectrometry
|June 27, 2015
PubMed
Summary
This summary is machine-generated.

Optimizing electron transfer dissociation (ETD) in mass spectrometry is crucial. This study introduces an automated calibration routine for ETD reaction duration, significantly improving peptide identification rates.

Keywords:
CalibrationETDIon/ionKineticsMass spectrometryOrbitrapProteomics

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.9K
Mapping Dysfunctional Protein-Protein Interactions in Disease
09:39

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

1.1K

Related Experiment Videos

Last Updated: Apr 8, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

10.2K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.9K
Mapping Dysfunctional Protein-Protein Interactions in Disease
09:39

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

1.1K

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Electron transfer dissociation (ETD) is a widely adopted technique in mass spectrometry.
  • Optimal ETD performance requires significant user expertise and parameter optimization.
  • Reaction duration is a critical factor influencing ETD spectral quality and experimental outcomes.

Purpose of the Study:

  • To develop and implement an automated calibration routine for optimizing ETD reaction duration.
  • To determine the precise number of reagent anions needed for a specific ETD reaction rate.
  • To enhance spectral quality and peptide identification efficiency in mass spectrometry experiments.

Main Methods:

  • Development of an automated calibration routine for ETD.
  • Quantification of reagent anions to achieve a defined ETD reaction rate.
  • Implementation and testing of the routine on hybrid Orbitrap mass spectrometers.

Main Results:

  • The automated calibration routine successfully optimized ETD reaction duration.
  • Implementation led to increased product ion yield.
  • Reduced scan rates were achieved, resulting in up to 75% more unique peptide identifications in shotgun proteomics experiments.

Conclusions:

  • The automated calibration routine offers significant advantages for ETD optimization.
  • This method enhances the efficiency and depth of peptide identification in mass spectrometry.
  • The routine provides a user-friendly approach to achieving optimal ETD performance.