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Published on: March 5, 2019
Angiotensin II directly impairs adipogenic differentiation of human preadipose cells
Marisol M Palominos1, Natalia H Dünner1, Martin Wabitsch2
1Faculty of Medicine, Institute of Biomedical Sciences, Universidad de Chile, Clasificador 7 Correo 7, Santiago, Chile.
Abstract:
Angiotensin II reduces adipogenic differentiation of preadipose cells present in the stroma-vascular fraction of human adipose tissue, which also includes several cell types. Because of the ability of non-adipose lineage cells in the stroma-vascular fraction to respond to angiotensin II, it is not possible to unequivocally ascribe the anti-adipogenic response to a direct effect of this hormone on preadipose cells. Therefore, we used the human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell strain to investigate the consequences of angiotensin II treatment on adipogenic differentiation under serum-free conditions, by assessing expression of typical adipocyte markers perilipin and fatty acid-binding protein 4 (FABP4), at the transcript and protein level. Reverse transcription-polymerase chain reaction showed that perilipin and FABP4 transcripts were, respectively, reduced to 0.33 ± 0.07 (P < 0.05) and 0.41 ± 0.19-fold (P < 0.05) in SGBS cells induced to adipogenic differentiation in the presence of angiotensin II. Western Blot analysis corroborated reduction of the corresponding proteins to 0.23 ± 0.21 (P < 0.01) and 0.46 ± 0.30-fold (P < 0.01) the respective controls without angiotensin II. Angiotensin II also impaired morphological changes associated with early adipogenesis. Hence, we demonstrated that angiotensin II is able to directly reduce adipogenic differentiation of SGBS preadipose cells.
Insights
Angiotensin II directly inhibits adipogenic differentiation in human preadipose cells. This hormone reduces key adipocyte marker expression at both transcript and protein levels, confirming its anti-adipogenic effect.
Area of Science:
- Cell Biology
- Endocrinology
- Metabolic Research
Background:
- Adipose tissue stromal-vascular fraction contains various cell types, including preadipocytes.
- Angiotensin II's effect on preadipose cells is difficult to isolate due to responses from other cell types.
- Simpson-Golabi-Behmel syndrome (SGBS) preadipocytes offer a model to study direct hormonal effects.
Purpose of the Study:
- To investigate the direct impact of Angiotensin II on adipogenic differentiation.
- To assess the effect of Angiotensin II on adipocyte marker expression in SGBS preadipocytes.
- To determine if Angiotensin II directly inhibits preadipose cell differentiation.
Main Methods:
- Utilized human SGBS preadipocyte cell strain under serum-free conditions.
- Assessed adipogenic differentiation by measuring perilipin and fatty acid-binding protein 4 (FABP4) expression.
- Quantified gene expression using reverse transcription-polymerase chain reaction (RT-PCR) and protein levels via Western Blot analysis.
Main Results:
- Angiotensin II significantly reduced perilipin and FABP4 transcript levels (0.33-fold and 0.41-fold, respectively).
- Western Blot analysis confirmed reduced perilipin and FABP4 protein levels (0.23-fold and 0.46-fold, respectively).
- Observed impaired morphological changes characteristic of early adipogenesis in the presence of Angiotensin II.
Conclusions:
- Angiotensin II directly inhibits the adipogenic differentiation of SGBS preadipose cells.
- The study provides direct evidence for Angiotensin II's anti-adipogenic role.
- Findings contribute to understanding hormonal regulation of adipose tissue development and metabolism.
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