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Related Experiment Videos

Phase tracking: an improved phase detection technique for cell membrane capacitance measurements.

N Fidler1, J M Fernandez

  • 1Department of Physiology, University of Pennsylvania, Philadelphia 19104-6085.

Biophysical Journal
|December 1, 1989
PubMed
Summary
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Phase tracking significantly enhances single-cell membrane capacitance measurements by automating phase angle adjustments. This new method accurately quantifies cellular exocytosis and endocytosis, even with changing cell properties.

Area of Science:

  • Cell biology
  • Electrophysiology
  • Biophysics

Background:

  • Accurate measurement of single-cell membrane capacitance is crucial for understanding cellular processes.
  • Existing methods for capacitance measurement can be limited by complex impedance matching requirements and drift in cell parameters.

Purpose of the Study:

  • To introduce and validate a novel technique, phase tracking, for improving the accuracy of single-cell membrane capacitance measurements.
  • To demonstrate the utility of phase tracking in quantifying dynamic cellular events like exocytosis and endocytosis.

Main Methods:

  • Modification of the original phase detection technique to incorporate calibrated changes in series resistance.
  • Automation of phase detector adjustment to the appropriate phase angle for capacitance measurement.

Related Experiment Videos

  • Application of phase tracking during measurements of exocytosis in mast cells, monitoring cell membrane resistance, capacitance, and series resistance.

    • Main Results:

    • Phase tracking allows for accurate determination of the capacitance signal's phase angle, independent of cell impedance matching.
    • The technique successfully maintains detector alignment with the capacitance signal despite significant drifts in cell electrical parameters.
    • Demonstrated accurate quantification of exocytosis in mast cells using the phase tracking method.

    Conclusions:

    • Phase tracking offers a robust and accurate method for measuring single-cell membrane capacitance.
    • This technique overcomes limitations of previous methods by automating phase angle adjustment and compensating for parameter drift.
    • Phase tracking is a valuable tool for quantifying exocytosis and endocytosis in real-time single-cell studies.