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Probing of DNA structure with osmium tetroxide. Effect of ligands
E Palecek1, P Boublíková, K Nejedlý
1Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Biophysical Chemistry
|September 15, 1989
Abstract:
Fourteen OsO4 complexes with different ligands were tested as probes of DNA structure. Of these complexes, only OsO4-2,2'-bipyridine (Os-bipy), OsO4-bathophenanthrolinedisulfonic acid (Os-bpds) and OsO4-N,N,N',N'-tetramethylenediamine (Os-TMEN) site-specifically modified the ColE1 cruciform in a supercoiled plasmid pColIR215 at millimolar concentrations. Os-bipy, Os-bpds and Os-TMEN also displayed site-specific modification of the B-Z junctions in the supercoiled plasmid pRW751 containing (dC-dG)n inserts.