Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic monitoring of antibody drug conjugates targeting TROP2 or HER2 in breast cancer using circulating tumor cells.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Inducing radiation resilience in frozen animal cells via mRNA coding for tardigrade damage-suppressor protein in support of space travel and Lunar storage.

The Journal of heredity·2026
Same author

Author Correction: HER2 expression identifies dynamic functional states within circulating breast cancer cells.

Nature·2026
Same author

Reduction in Red Blood Cell Lysis by Polymer Intervention During Rodent Liver Normothermic Machine Perfusion.

Transplantation direct·2026
Same author

Oscillatory flow for contactless particle trapping.

Lab on a chip·2026
Same author

Microfluidic automation improves oocyte recovery from follicular fluid of patients undergoing in vitro fertilization.

Nature medicine·2026

Related Experiment Video

Updated: Apr 8, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
09:27

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Published on: February 17, 2017

10.9K

Engineered Trehalose Permeable to Mammalian Cells.

Alireza Abazari1, Labros G Meimetis2, Ghyslain Budin2

  • 1The Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, Boston, Massachusetts, United States of America.

Plos One
|June 27, 2015
PubMed
Summary

Engineered trehalose derivatives enhance cellular protection. Trehalose hexaacetate efficiently enters cells and accumulates, offering a cell-friendly method for biopreservation applications.

More Related Videos

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
07:08

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Published on: December 23, 2015

14.4K
Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle
08:37

Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle

Published on: March 21, 2025

976

Related Experiment Videos

Last Updated: Apr 8, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
09:27

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Published on: February 17, 2017

10.9K
Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
07:08

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Published on: December 23, 2015

14.4K
Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle
08:37

Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle

Published on: March 21, 2025

976

Area of Science:

  • Biochemistry
  • Cell Biology
  • Biotechnology

Background:

  • Trehalose, a natural disaccharide, confers stress tolerance in organisms.
  • Mammalian cells lack natural mechanisms for trehalose synthesis or import.
  • Intracellular trehalose enhances tolerance to freezing and desiccation in mammalian cells.

Purpose of the Study:

  • To investigate novel methods for efficient intracellular delivery of trehalose into mammalian cells.
  • To evaluate the membrane permeability of engineered lipophilic trehalose derivatives.
  • To assess the potential of these derivatives for intracellular accumulation and biopreservation.

Main Methods:

  • Synthesis and testing of acetylated trehalose derivatives (trehalose diacetate, tetraacetate, hexaacetate).
  • Measurement of membrane permeability in rat hepatocytes.
  • Development of a diffusion-reaction model to describe intracellular accumulation kinetics.
  • Assessment of cellular viability and function post-trehalose loading.

Main Results:

  • Trehalose hexaacetate (6-O-Ac-Tre) showed superior cell membrane permeability compared to other derivatives and native trehalose.
  • Intracellular esterases hydrolyzed 6-O-Ac-Tre, releasing free trehalose.
  • Intracellular trehalose concentrations reached up to 10-fold the extracellular levels.
  • Trehalose loading had negligible impact on cellular viability and function.

Conclusions:

  • Engineering lipophilic trehalose derivatives provides an effective and cell-friendly strategy for intracellular trehalose delivery.
  • This method enables significant intracellular trehalose accumulation for biopreservation applications.
  • The approach avoids cellular manipulation, offering broad potential for various cell types.