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Amino acid analysis using chromatography-mass spectrometry: An inter platform comparison study.

P Krumpochova1, B Bruyneel2, D Molenaar3

  • 1AIMMS Division of BioAnalytical Chemistry, VU University Amsterdam, De Boelelaan 1083, 1081HV Amsterdam, The Netherlands; Systems Bioinformatics/AIMMS/NISB, VU University Amsterdam, De Boelelaan 1085, 1081HV Amsterdam, The Netherlands.

Journal of Pharmaceutical and Biomedical Analysis
|June 27, 2015
PubMed
Summary

This study compares three prominent amino acid analysis platforms: gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS) with derivatization, and hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS) of underivatized amino acids. All methods are suitable for complex biological samples, each with unique strengths and weaknesses for specific analytes.

Keywords:
Amino acidsGC–MSHILICLC–MS/MS

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