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The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

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Published on: January 7, 2022

Electroporation-based applications in biotechnology.

Tadej Kotnik¹, Wolfgang Frey², Martin Sack²

¹Department of Biomedical Engineering, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000 Ljubljana, Slovenia.

Trends in Biotechnology

|June 28, 2015

Summary

This summary is machine-generated.

Electroporation, a method using electric pulses, offers promising biotechnological applications beyond its medical uses. This review covers electrotransformation, microbial inactivation, biomolecule extraction, and biomass drying, including industrial and chip-scale uses.

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Area of Science:

Biotechnology and Biomedical Engineering
Microbiology
Biophysics

Background:

Electroporation is an established medical technique.
Emerging biotechnological applications are expanding its utility.
Understanding the phenomenon is key to its diverse applications.

Purpose of the Study:

To review promising emerging biotechnological applications of electroporation.
To highlight advancements in microbial genetic modification, inactivation, biomolecule extraction, and biomass drying.
To discuss both industrial-scale and chip-scale applications.

Main Methods:

Review of established and emerging electroporation techniques.
Analysis of applications including electrotransformation, microbial inactivation, biomolecule extraction, and biomass drying.
Exploration of industrial, clinical, and chip-scale implementations.

Main Results:

Electrotransformation is a well-established application for microbial genetic modification.
Recent advances show potential in microbial inactivation, biomolecule extraction, and rapid biomass drying.
Electroporation is applicable at both large industrial scales and small chip-scales.

Conclusions:

Electroporation presents significant potential for various biotechnological advancements.
Further research and development are needed to overcome challenges and realize future perspectives.
The versatility of electroporation spans from genetic engineering to material processing.