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Updated: Apr 8, 2026
Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method
Published on: February 24, 2023
Quantification of pollen tube attraction in response to guidance by female gametophyte tissue using artificial
Yoshikatsu Sato1, Nagisa Sugimoto1, Tetsuya Higashiyama2
1Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan.
Abstract:
We developed two types of artificial platforms, T-junction and crossroad microchannel devices, and obtained guidance response ratio of pollen tubes to the female tissue as 56-57%. The crossroad device was also able to collect the attracted pollen tubes with high purity, which is useful for future omics analysis.
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