Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

840
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
840
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

7.3K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
7.3K
Microbial Phylogeny01:28

Microbial Phylogeny

74
Understanding the evolutionary relationships among microorganisms is fundamental to microbial ecology and taxonomy. Phylogenetic trees are essential tools for inferring these relationships, relying primarily on comparative analyses of molecular sequences such as DNA, RNA, or proteins. In microbial studies, these trees typically depict the evolutionary paths of diverse bacterial and archaeal species by mapping genetic differences accumulated over time.Phylogenetic trees are composed of tips,...
74
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

665
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
665
Phylogenetic Species Concept in Microbiology01:22

Phylogenetic Species Concept in Microbiology

122
The phylogenetic species concept (PSC) is a framework used to delineate species based on evolutionary relationships, emphasizing shared ancestry and diagnosable genetic traits. Unlike morphological or biological species concepts, the PSC is particularly advantageous for microbial taxonomy, where traditional reproductive or phenotypic criteria often fall short due to the prevalence of asexual reproduction, minimal morphological differentiation, and widespread horizontal gene transfer among...
122
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

17.5K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
17.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Instruments to assess appropriateness of hip and knee arthroplasty: a systematic review.

Osteoarthritis and cartilage·2023
Same author

Public health genomics capacity assessment: readiness for large-scale pathogen genomic surveillance in Canada's public health laboratories.

BMC public health·2022
Same author

Identification of Reticulitermes Subterranean Termites (Blattodea: Rhinotermitidae) in the Eastern United States Using Inter-Simple Sequence Repeats.

Journal of economic entomology·2021
Same author

Pre-exposure prophylaxis for HIV infection and new sexually transmitted infections among men who have sex with men: real-life experience from three sexual health clinics in France.

Journal of the European Academy of Dermatology and Venereology : JEADV·2020
Same author

[Uveitis and tattoos].

Bulletin de l'Academie nationale de medecine·2020
Same author

Reducing Implant Infection in Orthopaedics (RIIiO): Results of a pilot study comparing the influence of forced air and resistive fabric warming technologies on postoperative infections following orthopaedic implant surgery.

The Journal of hospital infection·2019

Related Experiment Video

Updated: Apr 8, 2026

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

2.9K

Clostridium botulinum Group II Isolate Phylogenomic Profiling Using Whole-Genome Sequence Data.

K A Weedmark1, P Mabon1, K L Hayden1

  • 1National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.

Applied and Environmental Microbiology
|June 28, 2015
PubMed
Summary
This summary is machine-generated.

Whole-genome sequencing of Clostridium botulinum group II isolates revealed two distinct clusters. Core single nucleotide polymorphism (SNP) analysis offered higher resolution for classifying isolates and tracing outbreaks.

More Related Videos

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

9.1K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.1K

Related Experiment Videos

Last Updated: Apr 8, 2026

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

2.9K
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

9.1K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.1K

Area of Science:

  • Microbiology and Genomics
  • Food Safety and Public Health

Background:

  • Clostridium botulinum is a bacterium producing potent neurotoxins responsible for botulism.
  • Accurate characterization and typing of C. botulinum isolates are crucial for public health surveillance and outbreak investigations.

Purpose of the Study:

  • To characterize Clostridium botulinum group II isolates using in silico whole-genome sequence data.
  • To evaluate the discriminatory power of multilocus sequence typing (MLST) and core single nucleotide polymorphism (SNP) analysis for isolate classification and source attribution.

Main Methods:

  • Whole-genome sequencing (WGS) of 163 Clostridium botulinum group II isolates.
  • In silico analysis using multilocus sequence typing (MLST) and core single nucleotide polymorphism (SNP) analysis.
  • Comparison of typing methods for clade-level classification, isolate resolution, and outbreak/location discrimination.

Main Results:

  • Two distinct clusters of C. botulinum group II isolates were identified, encompassing various botulinum neurotoxin (BoNT) types and subtypes.
  • Core SNP analysis demonstrated higher resolution than MLST, accurately distinguishing isolates by outbreak and geographic origin.
  • Both MLST and core SNP analyses were consistent in clade-level classification.

Conclusions:

  • Next-generation sequencing-based typing approaches are valuable tools for C. botulinum isolate characterization and source attribution.
  • Core SNP analysis provides enhanced resolution for epidemiological investigations compared to traditional MLST.
  • Specific SNP loci and MLST alleles were identified for improved isolate comparison.