[Establishment of Human Acute B-Lymphoblastic Leukemia--NOD/SCID Xenotransplant Mouse Model]
Xin Zhang1, Yuan Deng1, Feng-Li Chen1
1Department of Hematology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
Objective:
To establish human B-acute lymphoblastic leukemia NOD/SCID mouse model.
Methods:
The unirradiated mice aged 4-5 weeks were injected with Nalm-6 cells via the tail vein. Successful transplantation was assessed by the general state, bone marrow smear and histopathologic examination.
Results:
After 15 days of injection with Nalm-6 cells, the NOD/SCID mice displayed the morbidily with lethargy, hind limb paralysis, hunched back and lossing weight. The infiltration of leukemia cells could be observed in bone marrow and spleen sections.
Conclusion:
Systemic B lineage acute leukemia animal mode1 has been successfully established in the NOD/SCID mice, which is a useful tool for studying pathogenesis of leukemia and guiding clinical chemotherapy regimens.
