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Updated: Apr 8, 2026

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis
Published on: May 1, 2015
[Correlation of WT1 and VEGF Expression with Angiogenesis in Bone Marrow Biopsies of Multiple Myeloma Patients]
Guo-Jie Li1, Hui Ding1, Liu Yang1
1Department of Hematology, Huaihe Hospital of Henan University, Kaifeng 475001, Henan Province, China.
Objective:
To investigate the relationship of WT1 and VEGF expression with angiogenesis in bone marrow biopsies of multiple myeloma patients.
Methods:
VEGF, WT1 expression and microvessel density (MVD) of 62 cases of multiple myeloma and 10 normal bone marrow tissue were detected by in situ hybridization and immunohistochemistry SP method.
Results:
Microvessel density (MVD) of the control group was (45±6)/visual field, and while MVD of the multiple myeloma group was (84±26)/sight, and statistical analysis showed that MVD in multiple myeloma group was significantly higher than that in control group (P<0.05); in 62 cases of multiple myeloma the VEGF positive rate was 51.6% (32/62), and MVD in VEGF-positive group was significantly higher than that in the negative group (P<0.05). WT1 positive rate was 30.6% (19/62), and MVD in WT1-positive group was significantly higher than that in the negative group (P<0.05). And statistical analysis showed that WT1 expression significantly correlated with VEGF expression (P<0.05).
Conclusion:
WT1 high expression of multiple myeloma bone tissue can up-regulate VEGF expression and promote angiogenesis.
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