Related Experiment Video
Updated: Apr 8, 2026
Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
Published on: July 5, 2021
Comparison of the single molecule activity distributions of recombinant and non-recombinant bovine intestinal
Douglas B Craig1, Fiona Hanlon-Dearman1, Shailah Beaudry1
1Chemistry Department, University of Winnipeg, Winnipeg, MB, Canada.
Abstract:
Single molecule assays were performed on bovine intestinal alkaline phosphatase and the recombinant enzyme expressed in Pichia pastoris using a capillary electrophoresis-based method. The catalytic rates for the bovine and recombinant enzymes were found to be 11,000±7000min(-1) (N=161) and 12,000±7000min(-1) (N=173), respectively. Mean catalytic rates and variances did not differ significantly between the enzyme from both sources. Furthermore, the distribution of catalytic rates were indistinguishable.
