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Related Experiment Videos

Bilayer membrane destabilization induced by dolichylphosphate.

J S Schutzbach1, J W Jensen

  • 1Department of Microbiology, University of Alabama, Birmingham 35294.

Chemistry and Physics of Lipids
|November 1, 1989
PubMed
Summary

Dolichyl phosphate (Dol-P) destabilizes phospholipid bilayers containing phosphatidylethanolamine (PE), causing leakage of encapsulated molecules. This effect is enhanced by divalent cations and is crucial for biological membrane processes.

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Area of Science:

  • Biochemistry
  • Membrane Biology
  • Lipid Chemistry

Background:

  • Dolichyl phosphate (Dol-P) is a lipid-linked oligosaccharide precursor.
  • Phospholipid bilayers form the structural basis of cell membranes.
  • Membrane stability is critical for cellular function and transport.

Purpose of the Study:

  • To investigate the impact of dolichyl phosphate (Dol-P) on phospholipid bilayer stability.
  • To determine the role of PE and PC phospholipids in Dol-P-mediated membrane destabilization.
  • To explore the relationship between Dol-P, membrane composition, and enzyme activity.

Main Methods:

  • Utilized small vesicles (liposomes) encapsulating the fluorescent probe calcein.
  • Assessed calcein leakage in response to divalent cations and varying temperatures.

Related Experiment Videos

  • Investigated the effect of adding rat liver Dol-P-mannose synthase to liposomes.

    • Main Results:

    • Dol-P induced calcein leakage from PE-containing phospholipid vesicles in the presence of divalent cations.
    • Leakage was dependent on divalent cation concentration and temperature, but not vesicle concentration.
    • Enzyme-induced leakage required both unsaturated PE and Dol-P, correlating with synthase activity.

    Conclusions:

    • Dolichyl phosphate significantly destabilizes phospholipid bilayers containing phosphatidylethanolamine.
    • This destabilization effect may play a role in the translocation of activated sugars across biological membranes.
    • The findings provide insights into the functional significance of Dol-P in membrane transport and lipid-protein interactions.