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Related Concept Videos

Gene Therapy00:59

Gene Therapy

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Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Modified-Release Drug Delivery Systems: Rate-Programmed I01:22

Modified-Release Drug Delivery Systems: Rate-Programmed I

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Rate-programmed drug delivery systems (DDS) are designed to release drugs at specific, controlled rates to maintain consistent therapeutic levels. These systems are categorized based on their release mechanisms, including dissolution-controlled DDS, diffusion-controlled DDS, and combined dissolution-diffusion-controlled DDS.In dissolution-controlled DDS, the release rate depends on the slow dissolution of the drug itself or the surrounding matrix. Drugs with inherently slow dissolution rates,...
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Modified-Release Drug Delivery Systems: Rate-Programmed II01:19

Modified-Release Drug Delivery Systems: Rate-Programmed II

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Rate-programmed drug delivery systems release drugs in a controlled manner to maintain therapeutic levels. Three main designs include reservoir, matrix, and hybrid systems.Reservoir systems consist of a drug core enclosed within a membrane that controls drug release. In non-swelling reservoir systems, polymers like ethyl cellulose or polymethacrylates are used. These do not hydrate in aqueous media and control release through membrane thickness, porosity, or insolubility. This type includes...
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Recombinant DNA01:09

Recombinant DNA

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Related Experiment Video

Updated: Apr 8, 2026

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
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How controlled release technology can aid gene delivery.

Jun-Ichiro Jo1, Yasuhiko Tabata1

  • 1a Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University , 53 Kawara-cho Shogoin, Sakyo-ku Kyoto 606-8507, Japan +81 75 751 4121 ; +81 75 751 4646 ; yasuhiko@frontier.kyoto-u.ac.jp.

Expert Opinion on Drug Delivery
|June 30, 2015
PubMed
Summary
This summary is machine-generated.

Controlled release technology using biodegradable biomaterials offers a feasible method for sustained gene delivery, enhancing gene expression and reducing adverse effects. Future applications include DNA hydrogels and exosomes for advanced gene therapy and regenerative medicine.

Keywords:
biodegradable biomaterialsdrug delivery systemintracellular controlled releaseregenerative medicine

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Area of Science:

  • Biomaterials Science
  • Gene Therapy
  • Regenerative Medicine

Background:

  • Gene delivery systems aim to enhance gene expression levels.
  • Controlled release technology offers sustained gene maintenance and release at injection sites.
  • Biodegradable biomaterials are key components for controlled release gene delivery systems, minimizing adverse effects from bolus administration and avoiding repeated dosing.

Purpose of the Study:

  • To review the role of controlled release technology in gene delivery for gene therapy and genetic engineering.
  • To highlight the importance of biodegradable biomaterials in developing effective gene delivery systems.
  • To discuss potential future applications of controlled release carriers in regenerative medicine.

Main Methods:

  • Review of controlled release-based gene delivery systems.
  • Discussion of biodegradable biomaterials for gene delivery.
  • Exploration of applications in gene therapy and genetic engineering.

Main Results:

  • Controlled release systems enable sustained therapeutic gene expression in gene therapy.
  • Intracellular controlled release from sub-micro-sized matrices is crucial for genetic engineering applications.
  • Biodegradable biomaterials facilitate the controlled release of genes from matrices.

Conclusions:

  • DNA hydrogels and exosomes show promise as controlled release carriers for therapeutic genes and individual-specific nucleic acids.
  • Advanced gene delivery technologies are essential for advancing stem cell biology, genetic engineering, and regenerative medicine.
  • Technologies for delivering genes to cell aggregates are critical for promoting regenerative research and therapy.