Related Experiment Video
Updated: Apr 8, 2026
Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
[Evaluation of the BD MGITTM technique for identifying the Mycobacterium tuberculosis complex]
Ingrid T Gómez1, Claudia R Llerena1, Angie P Zabaleta1
1Instituto Nacional de Salud Grupo Micobacterias, Bogotá, Colombia, tgomez@ins.gov.co.
Objectives:
To evaluate BD MGITTM TBc® technique for identifying the Mycobacterium tuberculosis complex using isolates obtained in liquid and solid media.
Methods:
A descriptive study was conducted in which 117 isolates were analyzed by the immune-chromatography technique obtained from solid and liquid cultures to identify the Mycobacterium tuberculosis complex. The kappa coefficient was calculated to determine the degree of agreement between the two methods .When there were different results, they were confirmed with a conventional test. The tool used to analyze the data was Epidat 3.1 RESULTS: The BD MGITTM TBc® methodology performed in solid and liquid culture isolates, showed an excellent degree of agreement with a kappa coefficient 0.84.
Conclusion:
The BD MGITTM TBc® technique using solid media culture isolates for the identification of the Mycobacterium tuberculosis complex has a good correlation compared to results obtained from liquid media culture isolates. The Reference National Laboratory recommends the use of this technique for the identification of species in solid media culture isolates.
