Related Experiment Video
Updated: Apr 8, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
Published on: June 29, 2017
Enhanced dipicolinic acid production during the stationary phase in Bacillus subtilis by blocking acetoin synthesis
Yoshihiro Toya1,2, Takashi Hirasawa1,2,3, Shu Ishikawa2,4
1a Department of Bioinformatic Engineering , Graduate School of Information Science and Technology, Osaka University , Suita , Japan.
Abstract:
Bacterial bio-production during the stationary phase is expected to lead to a high target yield because the cells do not consume the substrate for growth. Bacillus subtilis is widely used for bio-production, but little is known about the metabolism during the stationary phase. In this study, we focused on the dipicolinic acid (DPA) production by B. subtilis and investigated the metabolism. We found that DPA production competes with acetoin synthesis and that acetoin synthesis genes (alsSD) deletion increases DPA productivity by 1.4-fold. The mutant showed interesting features where the glucose uptake was inhibited, whereas the cell density increased by approximately 50%, resulting in similar volumetric glucose consumption to that of the parental strain. The metabolic profiles revealed accumulation of pyruvate, acetyl-CoA, and the TCA cycle intermediates in the alsSD mutant. Our results indicate that alsSD-deleted B. subtilis has potential as an effective host for stationary-phase production of compounds synthesized from these intermediates.
More Related Videos
09:27Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
Published on: June 24, 2016
10:17Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
Published on: October 9, 2016
Related Concept Videos
Production of Antibiotics
Production of Pharmaceuticals
Biosynthesis in Bacteria
Production of Organic Acids
Inhibitors of Gram-positive Cell Wall Synthesis
Amino Acid Biosynthetic Pathways