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Related Experiment Video

Updated: Apr 8, 2026

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

Published on: June 12, 2018

Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.

Ayca Yalcin Ozkumur¹, Brittany A Goods², J Christopher Love^3,4,5

¹Electrical and Electronics Engineering Department, Bahcesehir University, Istanbul, 34349, Turkey.

Small (Weinheim an Der Bergstrasse, Germany)

|June 30, 2015

Summary

This summary is machine-generated.

A new nanowell method enables cell-based drug discovery. This high-throughput assay identifies neutralizing antibodies against HIV, advancing functional therapeutic development.

Keywords:

cell patterning human immunodeficiency virus (HIV)living-cell arrays neutralization

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Last Updated: Apr 8, 2026

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Area of Science:

Biotechnology
Drug Discovery
Immunology

Background:

Living-cell-based screening is crucial for identifying functional therapeutic leads.
Existing methods may lack the scalability and precision needed for complex biological assays.

Purpose of the Study:

To develop a versatile and scalable method for cell-based lead discovery.
To establish a high-throughput, nanoliter-scale assay for identifying neutralizing antibodies.

Main Methods:

Utilizing dense arrays of nanowells to pattern cell monolayers.
Coordinating a multi-component biological assay within these nanowells.
Implementing a functional nanoliter-scale neutralization assay.

Main Results:

Demonstrated the ability to impart defined patterns on cell monolayers using nanowells.
Successfully coordinated a multi-component biological assay.
Identified neutralizing antibodies against HIV using the developed assay.

Conclusions:

The reported nanowell-based method is a versatile and scalable platform for cell-based screening.
This approach facilitates high-throughput identification of functional therapeutics, such as neutralizing antibodies against HIV.