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Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo
Published on: July 1, 2013
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Polycaprolactone-based in situ implant containing curcumin-PLGA nanoparticles prepared using the multivariate
Narayanan Kasinathan1, Muthukumar Amirthalingam1, Neetinkumar D Reddy1
1a Manipal College of Pharmaceutical Sciences, Manipal University , Manipal , Karnataka , India.
Artificial Cells, Nanomedicine, and Biotechnology
|June 30, 2015
Summary
Curcumin-PLGA nanoparticles (CPN) were optimized using multivariate analysis, showing sustained drug release and improved survival rates in cancer-bearing mice. The formulation demonstrated safety in vital organs, indicating therapeutic potential.
Area of Science:
- Nanotechnology
- Pharmacology
- Biomedical Engineering
Background:
- Curcumin exhibits anticancer properties but has poor bioavailability.
- Polymeric nanoparticles offer a promising delivery system for hydrophobic drugs like curcumin.
- Developing stable and effective curcumin-PLGA nanoparticles (CPN) is crucial for therapeutic applications.
Purpose of the Study:
- To optimize the formulation of curcumin-PLGA nanoparticles (CPN) using multivariate analysis.
- To evaluate the in vitro drug release profile of CPN.
- To assess the in vivo efficacy and safety of CPN in a murine cancer model.
Main Methods:
- Multivariate analysis was employed to study the effects of curcumin/PLGA ratio, PVA concentration, homogenization, and sonication parameters on particle size and drug encapsulation.
- In vitro drug release studies were conducted to determine the release kinetics of curcumin from CPN.
- Ehrlich ascites carcinoma (EAC)-bearing mice were used to evaluate the therapeutic efficacy and hematological effects of CPN.
- Histopathological examination of vital organs was performed to assess the safety of the CPN formulation.
Main Results:
- Particle size and drug encapsulation were significantly influenced by the interaction of sonication time with other formulation variables.
- CPN exhibited sustained release of curcumin.
- CPN administration significantly improved the survival rate of EAC-bearing mice.
- CPN effectively controlled EAC-induced changes in hematological parameters and showed no toxicity in vital organs.
Conclusions:
- The study successfully optimized CPN formulation using multivariate techniques, achieving desirable particle characteristics and high drug encapsulation.
- CPN demonstrated sustained curcumin release, enhanced anticancer efficacy, and a favorable safety profile in vivo.
- The developed CPN formulation holds significant potential for cancer therapy, with further investigation into in situ implants also showing promise.
