[Sodium Ferulate Attenuates Oxidative Stress Induced Inflammation via Suppressing NALP3 and NF-κB Signal Pathway]

To study the effects of sodium ferulate on inflammation in human lung epithelial cells (A549) under oxidative stress and itsinfluence onthe expression of inflammasome NACHT-PYD-containing protein 3 (NALP3) and nuclear factor kappa B (NF-κB).

Human lung epithelial cells A549 cultured in vitro were divided into 4 groups, including control group, H2O2 (100µmol/L) stress group, NF-κB blockers group (PDTC 100 µmol/L+ H2O2 100 µmol/L), sodium ferulate (SF) intervention group (SF 400µg/mL+ H2O2 10µmol/L). The expression of NALP3,IκBα protein were evaluated by Western blot, while mRNA levels of NALP3, NF-κB (P65) were measured by qRT-PCR. The level of interleukin-1beta (ILβ1) were detected by ELISA.

H2O2 not only increased the mRNA and protein expression levels of NALP3, but also enhanced the secretion of ILβ1p in human lung epithelial cells A549 (P<0. 05) when compared with control group. NF-κB blockers PDTC and sodium ferulateresisted the effects of H2O2 on A549 cells, that decreased the mRNA and protein expression of NALP3 and the mRNA expression of NF-κB (P65), reduced the degeneration of IκBα and the secretion of IL-1β (P<0. 05) when compared to H2O2 stress group.

SF may reduce the expression of NALP3 and IL-1β by inhibiting NF-κB, so as to reduce the inflammation caused by oxidative stress.