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Updated: Apr 8, 2026

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue
Published on: October 21, 2021
Development of the human fetal hippocampal formation during early second trimester
Xinting Ge1, Yonggang Shi2, Junning Li2
1Research Center for Sectional and Imaging Anatomy, Shandong Provincial Key Laboratory of Mental Disorders, Shandong University School of Medicine, 44 Wen-hua Xi Road, 250012 Jinan, Shandong, China; Laboratory of Neuroimaging, Institute of Neuroimaging and Informatics, Keck School of Medicine of University of Southern California, Los Angeles, CA 90033, USA.
Abstract:
Development of the fetal hippocampal formation has been difficult to fully describe because of rapid changes in its shape during the fetal period. The aims of this study were to: (1) segment the fetal hippocampal formation using 7.0 T MR images from 41 specimens with gestational ages ranging from 14 to 22 weeks and (2) reveal the developmental course of the fetal hippocampal formation using volume and shape analyses. Differences in hemispheric volume were observed, with the right hippocampi being larger than the left. Absolute volume changes showed a linear increase, while relative volume changes demonstrated an inverted-U shape trend during this period. Together these exhibited a variable developmental rate among different regions of the fetal brain. Different sub-regional growth of the fetal hippocampal formation was specifically observed using shape analysis. The fetal hippocampal formation possessed a prominent medial-lateral bidirectional shape growth pattern during its rotation process. Our results provide additional insight into 3D hippocampal morphology in the assessment of fetal brain development and can be used as a reference for future hippocampal studies.
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