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Preliminary method for direct quantification of colistin methanesulfonate by attenuated total reflectance Fourier

Krista L Niece1, Kevin S Akers2

  • 1Department of Extremity Trauma and Regenerative Medicine, United States Army Institute of Surgical Research, Fort Sam Houston, San Antonio, Texas, USA.

Antimicrobial Agents and Chemotherapy
|July 1, 2015
PubMed

Insights

Colistin methanesulfonate (CMS) is crucial for treating multidrug-resistant infections. A new method using ATR FTIR directly quantifies CMS, simplifying analysis compared to laborious traditional techniques.

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Pharmacology

Background:

  • Colistin use is rising due to multidrug-resistant organisms.
  • Colistin is administered as an inactive prodrug, colistin methanesulfonate (CMS).
  • Current CMS quantification methods are complex and time-consuming.

Purpose of the Study:

  • To develop a direct quantification method for colistin methanesulfonate (CMS).
  • To overcome limitations of existing UV-based HPLC methods for CMS analysis.

Main Methods:

  • Direct quantification of CMS using attenuated total reflectance Fourier transform infrared spectroscopy (ATR FTIR).
  • Avoidance of chemical conversion and precolumn derivatization steps.

Main Results:

  • Successful direct quantification of CMS was achieved using ATR FTIR.
  • The new method simplifies the analytical process for CMS.

Conclusions:

  • ATR FTIR offers a more efficient and direct approach for CMS quantification.
  • This method could improve the accuracy and ease of colistin dosing and pharmacokinetic studies.

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