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Updated: Jan 27, 2026

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides
Published on: November 2, 2021
Mass spectrometrists should search only for peptides they care about
1Department of Genome Sciences and the Department of Computer Science and Engineering, University of Washington, Seattle, Washington, USA.
Abstract:
Analysis pipelines that assign peptides to shotgun proteomics mass spectra often discard identified spectra deemed irrelevant to the scientific hypothesis being tested. To improve statistical power, I propose that researchers remove irrelevant peptides from the database prior to searching rather than assigning these peptides to spectra and then discarding the matches.
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