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Decreased microRNA-143 expression and its tumor suppressive function in human oral squamous cell carcinoma

Z Y Ni1, F O Lin1, D F Liu2

  • 1Department of Orthodontics, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.

Genetics and Molecular Research : GMR
|July 1, 2015
PubMed

Insights

MicroRNA-143 (miR-143) acts as a tumor suppressor in oral squamous cell carcinoma (OSCC). Its downregulation correlates with advanced disease and poor prognosis, suggesting miR-143 is a key regulator in OSCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-143 (miR-143) is a known tumor suppressor in various cancers.
  • The role of miR-143 in oral squamous cell carcinoma (OSCC) remains largely undetermined.

Purpose of the Study:

  • To investigate the expression and function of miR-143 in OSCC.
  • To determine the clinical significance and prognostic value of miR-143 in OSCC patients.

Main Methods:

  • Real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR) to measure miR-143 expression in 109 OSCC tissues and adjacent non-cancerous tissues.
  • Statistical analysis of miR-143 expression with clinicopathological factors and patient survival.
  • In vitro experiments to assess the impact of miR-143 upregulation on OSCC cell behavior (proliferation, invasion, migration, apoptosis).

Main Results:

  • miR-143 expression was significantly downregulated in OSCC tissues and cell lines compared to normal controls.
  • Lower miR-143 levels were significantly associated with advanced T classification, positive lymph node metastasis (N classification), advanced TNM stage, and reduced overall survival.
  • Restoring miR-143 expression in OSCC cells inhibited proliferation, invasion, and migration, while promoting apoptosis.

Conclusions:

  • miR-143 functions as a tumor suppressor in OSCC.
  • Downregulation of miR-143 is a significant indicator of poor prognosis in OSCC patients.
  • miR-143 holds potential as a therapeutic target and prognostic biomarker for OSCC.

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