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RhoBTB2 (DBC2) functions as a multifunctional tumor suppressor in thyroid cancer cells via mitochondrial apoptotic

Chuan-Jiang Wang1, Dong Yang1, Ying-Wei Luo1

  • 1Department of Vascular and Thyroid Surgery, The First Affiliated Hospital of China Medical University Nanjing North Street 155, Heping District, Shenyang City 110001, Liaoning Province, P. R. China.

International Journal of Clinical and Experimental Medicine
|July 2, 2015
PubMed

Insights

The tumor suppressor gene RhoBTB2 inhibits thyroid cancer cell growth by promoting apoptosis and damaging mitochondria. These effects were reversed by butin, an inhibitor of the mitochondrial apoptosis pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Thyroid cancer is the most common endocrine malignancy globally.
  • The tumor suppressor gene RhoBTB2 (Deleted in Breast Cancer 2, DBC2) is implicated in various carcinomas.
  • The role of RhoBTB2 in thyroid cancer remains unexplored.

Purpose of the Study:

  • To investigate the effects of RhoBTB2 on thyroid cancer cells.
  • To elucidate the underlying molecular mechanisms of RhoBTB2 action in thyroid cancer.

Main Methods:

  • Utilized SW579 thyroid cancer cells.
  • Assessed the impact of RhoBTB2 on cell proliferation, apoptosis, and mobility.
  • Analyzed protein expression of apoptosis-related factors (Bax, Bcl-2, Bcl-xL).
  • Investigated the role of the mitochondrial apoptosis pathway using butin.

Main Results:

  • RhoBTB2 significantly decreased proliferation and mobility while increasing apoptosis in SW579 cells.
  • RhoBTB2 induced mitochondrial damage, evidenced by altered Bax, Bcl-2, and Bcl-xL protein levels.
  • Butin treatment reversed the effects of RhoBTB2, indicating pathway involvement.

Conclusions:

  • RhoBTB2 acts as a tumor suppressor in thyroid cancer.
  • RhoBTB2 suppresses SW579 cell growth primarily through the mitochondrial apoptosis pathway.
  • Targeting RhoBTB2 or the mitochondrial apoptosis pathway may offer therapeutic strategies for thyroid cancer.
Keywords:
RhoBTB2apoptosismitochondrial apoptotic pathwaymobilitythyroid cancer

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