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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Apr 7, 2026

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

Assaying Cell Cycle Status Using Flow Cytometry.

Kang Ho Kim¹, Joel M Sederstrom²

¹Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas.

Current Protocols in Molecular Biology

|July 2, 2015

Summary

This summary is machine-generated.

This study presents two flow cytometry methods for cell cycle analysis, including Ki-67 staining and DNA/RNA differential staining. These techniques help identify quiescent cells and quantify cell cycle distribution for proliferation studies.

Keywords:

Hoechst 33342 Ki-67 Pyronin Y cell cycle flow cytometry propidium iodide

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Last Updated: Apr 7, 2026

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

Analysis of Cell Cycle Position in Mammalian Cells

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Area of Science:

Cell Biology
Biotechnology

Background:

Accurate cell cycle analysis is crucial for understanding cell proliferation and development.
Flow cytometry offers a powerful platform for quantitative cellular analysis.

Purpose of the Study:

To describe two distinct flow cytometry protocols for assessing cell cycle status.
To detail methods for identifying quiescent (G0) cells and quantifying cell cycle distribution (G1, S, G2/M).
To outline updated cell proliferation assays enabling multiplex analysis.

Main Methods:

Protocol 1: Simultaneous analysis of Ki-67 (proliferation marker) and DNA content.
Protocol 2: Differential staining of DNA and RNA using Hoechst 33342 and Pyronin Y.
Updated proliferation assays utilizing novel fluorophores for multiplexing.

Main Results:

Method 1 effectively discriminates G0 cells and quantifies cell cycle phases.
Method 2 differentiates G0 from G1 cells through DNA and RNA staining.
Newly developed fluorophores facilitate simultaneous assessment of cell cycle, proliferation, and gene expression.

Conclusions:

Established flow cytometry protocols provide robust methods for cell cycle analysis.
Advanced techniques allow for comprehensive, multiplexed cellular analysis in research and diagnostics.