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Related Concept Videos

Data Collection I01:30

Data Collection I

8.9K
Data collection gathers information needed to make accurate judgments about a patient's present condition. During a health history interview, subjective data is collected from the patient, their caregivers, or family members, and objective data is collected through observations and physical assessment. Patients are the primary source of subjective data. Thus information gathered from patients through interviews, observations, and physical examination is primary data. Secondary sources of...
8.9K
Data Collection by Observations01:08

Data Collection by Observations

15.7K
Data collection refers to a systematic way of obtaining, observing, measuring, and analyzing accurate information. Observational studies are one of the most widely used methods of data collection. It involves collecting data by observing the behavior and physical characteristics of a sample without making any modifications to the sample.
An astronomer viewing the motion and brightness of stars in the sky and recording the data is an example of observational data collection. A botanist recording...
15.7K
Data Collection III01:05

Data Collection III

4.8K
The physical assessment examines the patient for objective data that defines the patient's condition, and aids in formulating the nursing care plan. The purpose of physical assessment is a health status appraisal, which includes identifying health problems, and establishing a database for nursing intervention.
The principles to begin the physical assessment include conducting a comprehensive or problem-related history in a quiet, well-lit room, emphasizing privacy and comfort for the...
4.8K
Data Collection II01:29

Data Collection II

10.5K
The nursing history captures and records the patient's health status, so that a care plan evolves to meet the patient's individual needs. The nursing health history is a part of the initial assessment. A comprehensive history covers all health dimensions and plays a significant role in the assessment process. A comprehensive history includes the patient's biographical information, reasons for seeking health care, expectations, present and past health history, medications, and...
10.5K
Data Collection by Survey01:07

Data Collection by Survey

9.6K
The systematic method of obtaining and analyzing accurate information of a population is called data collection. A survey is a standard method of data collection that involves collecting information from a target human population about their experience, opinion, or knowledge of a product, service, or process. The responses are recorded and interpreted. The most common survey examples are written questionnaires, face-to-face or telephonic conversations, focus groups, and electronic (e-mail or...
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Data Collection by Experiments01:13

Data Collection by Experiments

28.1K
Data collection is a systematic method of obtaining, observing, measuring, and analyzing accurate information. An experimental study is a standard method of data collection that involves the manipulation of the samples by applying some form of treatment prior to data collection. It refers to manipulating one variable to determine its changes on another variable. The sample subjected to treatment is known as “experimental units.”
An example of the experimental method is a public...
28.1K

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Methodology for Establishing a Community-Wide Life Laboratory for Capturing Unobtrusive and Continuous Remote Activity and Health Data
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MicroED data collection and processing.

Johan Hattne1, Francis E Reyes1, Brent L Nannenga1

  • 1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.

Acta Crystallographica. Section A, Foundations and Advances
|July 2, 2015
PubMed
Summary
This summary is machine-generated.

Microcrystal electron diffraction (MicroED) determines atomic protein structures from tiny crystals, overcoming limitations of X-ray crystallography. This technique enables structural studies on previously intractable samples.

Keywords:
MicroEDcryo-EMcrystallographyelectron diffractionnanocrystals

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Area of Science:

  • Structural biology
  • Biophysics
  • Crystallography

Background:

  • X-ray crystallography requires large, well-ordered crystals, limiting its application.
  • Advances in electron cryo-microscopy and X-ray crystallography have driven MicroED development.
  • MicroED allows atomic structure determination from sub-micron crystals.

Purpose of the Study:

  • To detail MicroED methodology for atomic-resolution protein structure determination.
  • To highlight MicroED's potential in overcoming crystal size limitations.
  • To present unique challenges associated with MicroED.

Main Methods:

  • Sample preparation for MicroED analysis.
  • Data collection strategies for electron diffraction.
  • Data reduction and refinement for 3D model generation.

Main Results:

  • Successful determination of atomic protein structures using MicroED.
  • Demonstration of MicroED's capability with sub-micron crystals.
  • Identification of key steps and challenges in the MicroED workflow.

Conclusions:

  • MicroED is a powerful technique for atomic-resolution structural studies.
  • It overcomes crystal size limitations inherent in X-ray crystallography.
  • Further development can expand its application to difficult-to-crystallize proteins.