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Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
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Structure and Function of Cu(I)- and Zn(II)-ATPases

Oleg Sitsel1, Christina Grønberg2, Henriette Elisabeth Autzen2

  • 1Centre for Membrane Pumps in Cells and Disease (PUMPkin), Danish National Research Foundation, Department of Molecular Biology and Genetics, Aarhus University , Gustav Wieds Vej 10C, DK-8000 Aarhus C, Denmark.

Biochemistry
|July 2, 2015
PubMed
Summary

Copper and zinc are vital micronutrients, but toxic in excess. P-type ATPases (subclass 1B) maintain metal homeostasis by transporting copper and zinc ions across cell membranes. This study compares these ATPases to understand their distinct metal transport mechanisms.

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