Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chromosomal localization of DBL oncogene sequences.

S R Tronick1, O W McBride, N C Popescu

  • 1Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.

Genomics
|October 1, 1989
PubMed
Summary

The DBL oncogene arises from genomic rearrangements involving chromosomes X, 3, and 16. These findings indicate that the DBL and MCF2 oncogenes originate from the same genetic locus on the X chromosome.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of extracorporeal shock wave on proliferation and differentiation of equine adipose tissue-derived mesenchymal stem cells in vitro.

American journal of stem cells·2013
Same author

DLC1 induces expression of E-cadherin in prostate cancer cells through Rho pathway and suppresses invasion.

Oncogene·2013
Same author

A novel isoform of the 8p22 tumor suppressor gene DLC1 suppresses tumor growth and is frequently silenced in multiple common tumors.

Oncogene·2011
Same author

Mechanisms of bradykinin-induced contraction in human fetal lung fibroblasts.

The European respiratory journal·2010
Same author

p120Ras-GAP binds the DLC1 Rho-GAP tumor suppressor protein and inhibits its RhoA GTPase and growth-suppressing activities.

Oncogene·2009
Same author

DLC1 tumor suppressor gene inhibits migration and invasion of multiple myeloma cells through RhoA GTPase pathway.

Leukemia·2008

Area of Science:

  • Genetics
  • Oncology
  • Molecular Biology

Background:

  • The DBL oncogene is implicated in cancer development.
  • Understanding the genomic origin of oncogenes is crucial for cancer research.

Purpose of the Study:

  • To elucidate the genomic structure and chromosomal origin of the DBL oncogene.
  • To investigate the relationship between the DBL and MCF2 oncogenes.

Main Methods:

  • Human X rodent somatic cell hybrid analysis to map gene locations.
  • In situ hybridization for precise chromosomal localization.
  • Restriction mapping and nucleotide sequencing of genomic and cDNA clones.

Main Results:

  • The DBL proto-oncogene is located on the X chromosome (bands q26-27.2).

Related Experiment Videos

  • DBL resulted from recombination between the X chromosome and sequences from chromosomes 3 and 16.
  • DBL was localized to Xq27-q28 via in situ hybridization.
  • The MCF2 oncogene shares sequences from Xq27.
  • Restriction maps and sequences indicate DBL and MCF2 derive from the same locus.

    • Conclusions:

    • The DBL oncogene is formed through complex genomic rearrangements.
    • DBL and MCF2 oncogenes originate from a common genetic locus on the X chromosome, suggesting a shared evolutionary or functional relationship.