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The production of highly specific anti-testosterone antisera using acid-treated bacteria as immunogenic carrier

J M Cilliers1, H W Stindt, D U Bellstedt

  • 1Department of Biochemistry, University of Stellenbosch, Republic of South Africa.

Immunological Investigations
|November 1, 1989
PubMed

A novel and effective procedure for the production of highly specific anti-testosterone antibodies is described. It involved pretreatment of experimental animals with tolerogens followed by immunization with conjugates of testosterone covalently linked to acid-treated Salmonella minnesota R595 bacteria as immunogenic carriers. Antibodies elicited by this procedure showed minimal cross-reactivity towards 5 alpha-dihydrotestosterone and some of them were successfully used in radioimmunoassays for the determination of serum testosterone levels, even without the need for prior extraction.

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