Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Generation of Action Potential in Skeletal Muscles01:24

Generation of Action Potential in Skeletal Muscles

10.8K
Every cell in the body maintains a membrane potential due to an uneven distribution of positive and negative charges across its plasma membrane. The membrane potential is measured in millivolts and quantifies the difference in charge across the membrane.
Like neurons, muscle cells are also regarded as excitable due to their capacity to change in response to stimuli, primarily due to voltage-gated ion channels embedded in their plasma membranes, which get activated by alterations in the...
10.8K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

4.1K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
4.1K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.7K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.7K
Yeast Signaling01:28

Yeast Signaling

18.5K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
18.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic variation across the human olfactory receptor repertoire alters odor perception.

Proceedings of the National Academy of Sciences of the United States of America·2019
Same author

Whole exome sequencing and functional studies identify an intronic mutation in TRAPPC2 that causes SEDT.

Clinical genetics·2013
Same author

Acoustic cavitation, bubble dynamics and sonoluminescence.

Ultrasonics sonochemistry·2007
Same author

Analysis of the Zebrafish perplexed mutation reveals tissue-specific roles for de novo pyrimidine synthesis during development.

Genetics·2005
Same author

Transplantation procedures in social awareness: an opinion poll of Silesian Province citizens on cadaveric organ transplantation.

Transplantation proceedings·2003
Same author

Onset and time course of apoptosis in the developing zebrafish retina.

Cell and tissue research·2001
Same journal

Comprehensive Analysis of Auditory Nerve Fiber Responses using Fiber-Specific Modeling.

Journal of neurophysiology·2026
Same journal

HCN channels modulate the medium afterhyperpolarization and adjust the firing gain of fast alpha motoneurons in mice.

Journal of neurophysiology·2026
Same journal

Targeting intracranial electrical stimulation to network regions defined within individuals causes network-level effects.

Journal of neurophysiology·2026
Same journal

When "Noise" Isn't Simply Noise: Deterministic Postural Drive During Noisy Galvanic Vestibular Stimulation (nGVS).

Journal of neurophysiology·2026
Same journal

Abrupt Scene Onsets and Gradually Emerging Scene Information Produce Distinct EEG Decoding Dynamics.

Journal of neurophysiology·2026
Same journal

From discovery to translation: charting a course for the <i>Journal of Neurophysiology</i>.

Journal of neurophysiology·2026
See all related articles

Related Experiment Video

Updated: Apr 7, 2026

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
08:24

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

Published on: April 18, 2017

12.2K

pigk Mutation underlies macho behavior and affects Rohon-Beard cell excitability.

V Carmean1, M A Yonkers1, M B Tellez2

  • 1Neuroscience Program, University of Colorado Anschutz Medical Campus, Aurora, Colorado; Department of Physiology and Biophysics, University of Colorado Anschutz Medical Campus, Aurora, Colorado;

Journal of Neurophysiology
|July 3, 2015
PubMed
Summary
This summary is machine-generated.

A mutation in the pigk gene causes touch insensitivity in zebrafish by affecting sensory neuron function. Restoring pigk expression rescues touch response, revealing its crucial role beyond neuronal excitability.

Keywords:
NavPigkRohon-Beard cellsglycophosphatidylinositol-anchored proteintouch response

More Related Videos

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
07:14

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish

Published on: August 16, 2018

8.3K
A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster
05:48

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

Published on: December 30, 2015

10.7K

Related Experiment Videos

Last Updated: Apr 7, 2026

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
08:24

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

Published on: April 18, 2017

12.2K
Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
07:14

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish

Published on: August 16, 2018

8.3K
A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster
05:48

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

Published on: December 30, 2015

10.7K

Area of Science:

  • Neuroscience
  • Genetics
  • Cell Biology

Background:

  • Touch-evoked behavior relies on sensory neuron function.
  • The zebrafish mutant 'macho' exhibits touch insensitivity and impaired neuronal sodium currents.

Purpose of the Study:

  • To identify the genetic basis of the 'macho' zebrafish touch-insensitive phenotype.
  • To elucidate the role of the identified gene in sensory neuron function and touch response.

Main Methods:

  • Whole-genome sequencing to identify mutations in 'macho' zebrafish.
  • Pigk gene knockdown and overexpression experiments in zebrafish embryos.
  • Electrophysiological recordings of sensory neuron sodium currents (INa).
  • Behavioral assays to assess touch response.

Main Results:

  • A mutation in the pigk gene was identified in 'macho' mutants.
  • PigK knockdown phenocopied 'macho' touch insensitivity and reduced INa.
  • Overexpression of wild-type pigk rescued touch response and neuronal function in mutants.
  • Pigk's role extends beyond maintaining INa and action potential firing for behavioral touch response.

Conclusions:

  • The pigk gene is essential for touch-evoked behavior in zebrafish.
  • PigK function is critical for sensory neuron sodium currents and action potential generation.
  • Pigk plays a vital role in touch response independent of its function in neuronal excitability.