Related Experiment Video
Updated: Apr 7, 2026
PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS
Published on: December 27, 2013
PLGA-Based Nanoparticles: a Safe and Suitable Delivery Platform for Osteoarticular Pathologies.
Mathieu Riffault1, Jean-Luc Six2, Patrick Netter1
1Ingénierie Moléculaire et Physiopathologie Articulaire, Unité Mixte de Recherche 7365, Centre National de la Recherche Scientifique - Université de Lorraine, Biopôle de l'Université de Lorraine, Campus Biologie Santé, 9 Avenue de la Forêt de Haye CS 50184, 54505, Vandœuvre Lès Nancy cedex, France.
Poly(lactic-co-glycolic)-acid (PLGA) nanoparticles are well-tolerated in joints. These particles showed minimal impact on cartilage cells and joint structure after intra-articular injection in rats.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Rheumatology
Background:
- Poly(lactic-co-glycolic)-acid (PLGA) nanoparticles offer promising applications for intra-articular drug delivery.
- Limited data exists on the biocompatibility and fate of PLGA nanoparticles within the joint environment post-administration.
Purpose of the Study:
- To evaluate the biocompatibility of unloaded PLGA-based nanoparticles following intra-articular administration.
- To assess the impact of these nanoparticles on articular cell types and joint structure.
- To develop a fluorescently labeled PLGA nanoparticle with a hyaluronate shell for enhanced cell tracking and recognition.
Main Methods:
- Fluorescent microscopy was used to track nanoparticle internalization into cells over time.
- Quantitative real-time PCR (RT-qPCR) and biochemical assays monitored inflammatory responses.
- Mesenchymal stem cells (MSCs) were assessed for differentiation potential (chondrogenic, adipogenic, osteogenic) after nanoparticle exposure.
- Histological analysis of rat knee joints evaluated structural integrity after intra-articular injections.
Main Results:
- Nanoparticles were observed within the cytoplasm of cells within 8 hours of exposure.
- A minor, reversible increase in inflammatory markers was noted, less than that observed in inflammatory conditions.
- Nanoparticle exposure demonstrated minimal impact on MSC pluripotency and differentiation capabilities.
- Histological examination revealed no adverse effects on joint structures following intra-articular administration.
Conclusions:
- The developed PLGA-based nanoparticle delivery system is well-tolerated within the joint environment.
- This platform shows potential for the localized delivery of therapeutic agents to the joint.
