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Updated: Aug 7, 2026
Porous Silicon Microparticles for Delivery of siRNA Therapeutics
Published on: January 15, 2015
Chitosan-coated red fluorescent protein nanoparticle as a potential dual-functional siRNA carrier
Ying Liu1, Zheng-Mei Song1, Xiaoyong Deng1
1Institute of Nanochemistry & Nanobiology, Shanghai University, Shanghai, 200444, China.
Aims:
Developing safe and efficient nano vectors is critical for the success of siRNA therapy.
Materials & Methods:
By encapsulating red fluorescent protein (RFP) with chitosan (CS), a dual-functional siRNA delivery nano vector, RFP@CS, has been synthesized.
Results:
RFP@CS has an optimum size of 7-23 nm for siRNA delivery; and the fluorescence of RFP, protected by CS coating, provides an excellent probe to track the delivery of siRNA. RFP@CS delivers siRNA efficiently into cells and the targeted gene could be completely silenced even after 48 h. No cytotoxicity or acute toxicity in mice was observed.
Conclusion:
The high transfection efficacy and safety demonstrate RFP@CS is a promising nano vector for the gene therapy.
