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Related Experiment Video

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Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
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Decrease in fluorescence lifetime by glycation of collagen and its application in determining advanced glycation

Shuichiro Fukushima1, Masato Shimizu2, Jiro Miura3

  • 1Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Japan ; These authors contributed equally to the work.

Biomedical Optics Express
|July 3, 2015
PubMed
Summary

Advanced Glycation End-products (AGEs) form via the Maillard reaction, altering collagen. Fluorescence lifetime measurement effectively quantifies AGEs in collagen tissues, as shown in human dentin.

Keywords:
(000.1430) Biology and medicine(030.5260) Photon counting(170.1850) Dentistry(170.2520) Fluorescence microscopy(170.3650) Lifetime-based sensing

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Area of Science:

  • Biochemistry
  • Biomaterials Science
  • Biophysics

Background:

  • Advanced Glycation End-products (AGEs) are formed through the Maillard reaction.
  • Collagen cross-linking by AGEs alters the mechanical properties of tissues.
  • Fluorescence measurement is a viable method for AGE detection.

Purpose of the Study:

  • To investigate fluorescence lifetime measurement (FLM) as a method for quantifying AGEs.
  • To assess the effect of glycation on the fluorescence lifetime of collagen.
  • To validate the in vivo application of FLM for AGE determination in human dentin.

Main Methods:

  • Utilized fluorescence lifetime measurement (FLM) to analyze collagen.
  • Compared FLM with steady-state fluorometry, noting limitations of the latter.
  • Applied FLM to in vivo samples of human dentin.

Main Results:

  • Collagen fluorescence lifetime was found to decrease with increasing glycation.
  • FLM demonstrated effectiveness in tracking glycation progress in collagen gels.
  • Successful in vivo application of FLM for AGE determination in human dentin was confirmed.

Conclusions:

  • Fluorescence lifetime measurement is a robust technique for quantifying AGEs in collagen.
  • FLM offers advantages over traditional fluorometry due to its independence from sample surface conditions.
  • FLM shows promise for non-invasive assessment of AGEs in biological tissues.