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Related Experiment Videos

Saccharide expression on wounded endothelial cell monolayers in vitro.

R Y Ball1, R W Stoddart, C J Jones

  • 1Department of Pathology, University of Cambridge, UK.

Journal of Cell Science
|May 1, 1989
PubMed
Summary

Endothelial cells respond to injury by spreading, migrating, and proliferating. This process involves specific cell-surface saccharides, potentially aiding monocyte adhesion and migration in large arteries.

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Area of Science:

  • Endothelial cell biology
  • Vascular injury response
  • Cellular adhesion mechanisms

Background:

  • The endothelium lining large arteries is crucial for vascular health.
  • Understanding endothelial cell responses to injury is vital for preventing atherosclerosis and other vascular diseases.
  • Monocyte interaction with the endothelium is a key event in inflammatory responses.

Purpose of the Study:

  • To investigate the sequence of cellular events following injury in a model of large artery endothelium.
  • To identify molecular changes, specifically saccharide expression, associated with endothelial cell spreading after injury.
  • To explore the potential role of these molecular changes in monocyte recruitment.

Main Methods:

  • Utilized primary porcine aortic endothelial cells in monolayer culture.

Related Experiment Videos

  • Induced linear wounds via scraping to mimic endothelial injury.
  • Analyzed cellular spreading, migration, and proliferation over time.
  • Investigated cell surface saccharide expression using lectin binding (ConA, LCA, PSA) and enzyme sensitivity (alpha-mannosidase).

    • Main Results:

    • Observed a temporal sequence: cellular spreading (2h), migration (6-8h), and proliferation (16h+).
    • Identified the appearance of specific saccharides at spreading cell margins.
    • These saccharides bound ConA, LCA, and PSA lectins and were sensitive to alpha-mannosidase, indicating terminal alpha-mannosyl residues.
    • The presence of these residues suggests a mechanism for monocyte interaction.

    Conclusions:

    • Endothelial cells exhibit a coordinated response to injury involving spreading, migration, and proliferation.
    • Specific mannose-containing saccharides appear at the cell periphery during spreading.
    • These saccharides may mediate monocyte adhesion and transmigration at sites of vascular injury.
    • This finding provides insight into early inflammatory events in large arteries.