[Comparison for ELISA and CLIA of serum 25-hydroxy vitamin D determination]

To estimate the bias between the enzyme linked immunosorbent assay (ELISA) and chemiluminescence immunoassay (CLIA) of serum 25-hydroxy vitamin D (25 (OH) D) determination, and to evaluate the correspondence of two methods.

Forty human serum samples were collected, and the serum 25 (OH) D were determined by immunodiagnostic systems 25-hydroxy vitamin D EIA and immunodiagnostic systems iSYS analyser. According to the standards of NCCLS EP9-A2 files, the within and without outlier, test adequate range, used linear regression procedure, and compare average and maximum bias between these two methods were excluded.

In the linear regression analysis, the results of the two methods were correlated, y = 0.995x + 2.3928, r = 0.981. The relative standard deviation between these two methods was smaller with the higher serum 25(OH) D levels.

The correlation and repeatability of serum 25 (OH) D determination by ELISA and CLIA are fine.